Novel motor proteins and methods for their use
OwnerChristophe Beruad, Roman Sakowicz +1inventors
InventorsChristophe Beruad, Roman Sakowicz, Kenneth W. Wood
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Abstract
The invention provides isolated nucleic acid and amino acid sequences of HsKif15, antibodies to HsKif15, methods of screening for HsKif15 modulators using biologically active HsKif15, and kits for screening for HsKif15 modulators.
Claims19
An isolated nucleic acid sequence encoding a microtubule motor protein, wherein the motor protein has the following properties:
- (i) the protein's activity includes microtubule stimulated ATPase activity;
- and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:
- 2 as measured using a sequence comparison algorithm.
An expression vector comprising a nucleic acid encoding a microtubule motor protein, wherein the motor protein has the following properties:
- (i) the protein's activity includes microtubule stimulated ATPase activity;
- and (ii) the protein has a sequence that has greater than 70% amino acid sequence identity to SEQ ID NO:
- 2 as measured using a sequence comparison algorithm.
An isolated microtubule motor protein, wherein the protein has greater than 70% amino acid sequence identity to SEQ ID NO:
- 4 or SEQ ID NO:
- 6 as measured using a sequence comparison algorithm.
A method for screening for modulators of HsKif15, the method comprising the steps of:
- (i) providing biologically active HsKif15, wherein has the following properties:
- (i) activity including microtubule stimulated ATPase activity;
- and (ii) sequence that has greater than 70% amino acid sequence identity to HsKif15 of SEQ ID NO:
- 2 as measured using a sequence comparison algorithm;
- (ii) contacting biologically active HsKif15 with a candidate agent in a test and control concentration;
- and
- (iii) assaying for the level of HsKif15 activity, wherein the HsKif15 activity is selected from the group consisting of binding activity or ATPase activity, and wherein a change in activity between the test and control concentration indicates a modulator.
A kit for screening for modulators of HsKif15, the kit comprising;
- (i) a container holding biologically active HsKif15;
- and
- (ii) instructions for assaying for HsKif15 activity, wherein the HsKif15 activity is microtubule binding activity, or microtubule stimulated AT~ase activity.
An isolated nucleic acid comprising a sequence which has greater than 60% sequence identity with nucleotide SEQ ID NO:
- 1, SEQ ID NO:
- 3 or SEQ ID NO:
- 5.
Dependencies are inferred from the claim text.