Soluble chimeric G protein alpha subunits
OwnerStephen G. Graberinventor
Life of this patent
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Abstract
The present invention provides a chimeric α subunit of G proteins having n (n=3 to 50) amino acids from the N-terminus and c (c=3 to 50) amino acids from the C-terminus of a donor α subunit of a G protein at the N-terminus and C-terminus of the chimeric α subunit, wherein the internal part of the chimeric α subunit is a recipient α subunit which is the α subunit of a G protein of a different subclass than the donor α subunit having m amino acids and/or d amino acids optionally removed from the N-terminus and C-terminus, respectively, of the recipient α subunit, wherein m is n−10, n−9, n−8, n−7, n−6, n−5, n−4, n−3, n−2, n−1, n, n+1, n+2, n+3, n+4, n+5, n+6, n+7, n+8, n+9 or n+10 and d is c-10, c-9, c-8, c-7, c-6, c-5, c-4, c-3, c-2, c-1, c, c+1, c+2, c+3, c+4, c+5, c+6, c+7, c+8, c+9 or c+10.
Claims29
A chimeric α subunit of G proteins, which chimeric α subunit is represented by formula (I):
- ti B1—B2—B3 (I) PS
- wherein
- B1 is the N-terminus and B3 is the C-terminus of the chimeric α subunit;
- B1 is a peptide having the N-terminal amino acid sequence of n amino acids in length from donor alpha, wherein donor alpha is an α subunit of a donor G protein;
- B3 is a peptide having the C-terminal amino acid sequence of c amino acids in length from donor alpha;
- B2 is selected from the group consisting of (A) recipient alpha, which is an α subunit of a recipient G protein different from the α subunit of the donor G protein, (B) recipient alpha minus n−10, n−9, n−8, n−7, n−6, n−5, n−4, n−3, n−2, n−1, n, n+1, n+2, n+3, n+4, n+5, n+6, n+7, n+8, n+9 or n+10 consecutive amino acid residues from the N-terminus, (C) recipient alpha minus c−10, c−9, c−8, c−7, c−6, c−5, c−4, c−3, c−2, c−1, c, c+1, c+2, c+3, c+4, c+5, c+6, c+7, c+8, c+9 or c+10 consecutive amino acid residues from the C-terminus, and (D) recipient alpha minus n−10, n−9, n−8, n−7, n−6, n−5, n−4, n−3, n−2, n−1, n, n+1, n+2, n+3, n+4, n+5, n+6, n+7, n+8, n+9 or n+10 consecutive amino acid residues from the N-terminus and minus c−10, c−9, c−8, c−7, c−6, c−5, c−4, c−3, c−2, c−1, c, c+1, c+2, c+3, c+4, c+5, c+6, c+7, c+8, c+9 or c+10 consecutive amino acid residues from the C-terminus;
- wherein
- n and c are independently about 3 to about 50, about 3 to about 44, about 3 to about 42, about 3 to about 40, about 3 to about 38, about 3 to about 35, about 6 to about 50, about 6 to about 44, about 6 to about 42, about 6 to about 40, about 6 to about 38, about 6 to about 35, about 6 to about 32, about 6 to about 30, about 8 to about 38, about 8 to about 35, about 8 to about 32, about 8 to about 30, about 10 to about 32, about 10 to about 30, about 10 to about 28, about 10 to about 25, about 12 to about 25 or about 12 to about 20 amino acid residues;
- n and c can be the same or different;
- and
- B1 and B2, and B2 and B3, are linked with a peptide bond.
Dependencies are inferred from the claim text.