Pasteurella neuraminidase coding sequences, compositions and diagnostic methods
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsMARGIE LEE, SUSAN SANCHEZ, ADAM HENK
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Abstract
Provided are Pasteurella multocida coding sequences, recombinant DNA molecules, recombinant host cells and methods for making recombinant neuraminidase. Also provided by the present disclosure are immunogenic compositions containing recombinant P. multocida neuraminidase and antigenic peptides derived in sequence therefrom and antibodies specific for P. multocida neuraminidase as well as immunoassays specific for P. multocida neuraminidase, which immunoassays are useful in the detection or diagnosis of P. multocida infections and/or carrier states.
Claims7
An immunogenic composition comprising a recombinant neuraminidase derived from Pasteurella multocida, wherein said recombinant neuraminidase has at least about 80% amino acid sequence identity with SEQ ID NO:5, amino acids 1-412 or comprising at least one immunogenic peptide derived in sequence from said neuraminidase.
An immunogenic composition for generating an immune response to a neuraminidase of P. multocida, said composition comprising one or more immunogenic peptides having amino acid sequences as given in SEQ ID NO:1, SEQ ID NO:2 OR SEQ ID NO:3 together with an excipient.
An immunoassay kit for the diagnosis and/or detection of Pasteurella multocida in a biological sample from an animal or human, said immunoassay kit comprising antibody specific for neuraminidase from Pasteurella multocida, and reagents for detection of specific binding of Pasteurella multocida neuraminidase to the antibody provided.
A method for detecting Pasteurella multocida within an animal or human, said method comprising the steps of:
- (a) obtaining at least one biological sample from an animal or human;
- (b) reacting the sample of step (a) with the immunoassay kit reagents of claim 18;
- and
- (c) detecting the presence or absence of specific binding of neuraminidase within the sample, if present, to antibody specific for P. multocida neuraminidase, whereby Pasteurella multocida is detected or diagnosed when a specific binding is detected in step (c).
Dependencies are inferred from the claim text.