US 2004/0072151 A1 · Method of extracting virus genes and extraction apparatus

Abstract

The present invention provides a method of extracting virus genes, which is excellent in quickness and convenience and can be automated, and this invention also provides an apparatus for such extraction of virus genes. A conductive hollow fiber membrane (module) 12 is immersed in a virus suspension 20 and thus viral particles suspended in the virus suspension 20 are captured in the conductive hollow fiber membrane (module) 12. The coating of the thus captured virus is lysed and the virus genes are released. Next, the virus genes are collected by subjecting the conductive hollow fiber membrane (module) 12 to at least one of an electrical treatment and a physical treatment.

Claims12

Dependent claims

A method of extracting virus genes from a virus suspension, comprising the steps of:

immersing a conductive hollow fiber membrane in the virus suspension and thereby capturing, in the conductive hollow fiber membrane, viruses suspended in the virus suspension;
lysing the coating of the captured viruses and releasing the virus genes;
and
collecting the virus genes by subjecting, after the lysing step, the conductive hollow fiber membrane to at least one of an electrical treatment and a physical treatment.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

10/469,737Filed 14 Nov 2003

Priority

JP 2001-638347 Mar 2001

Published

15 Apr 2004

Term ends

Not estimatedThe source records don’t support an estimate.

Classification4 codes

C12N 15/1017
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12N
Microorganisms or enzymes
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Method of extracting virus genes and extraction apparatus

Life of this patent

Method of extracting virus genes and extraction apparatus

Life of this patent

Abstract

Claims12