Method and apparatus for isolation and purification of biomolecules
AssigneeNew Jersey Institute of Technology
InventorsKamalesh K. Sirkar, Robert G. Luo, Yanke Xu, Xiao-Ping Dai
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Abstract
An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane means disposed in the housing for filtering the bioproducts from the feed solution wherein a portion of the membrane means is coated with a polymeric coating, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.
Claims51
A membrane module means for recovering bioproducts from a feed solution, said means comprising a module housing, membrane means disposed in said housing for filtering the bioproducts from the feed solution wherein a portion of the feed side of the membrane means is coated with a polymeric coating so as to render such portion impermeable to the bioproducts to be recovered, and an adsorbent bed means disposed in said housing for retaining the bioproducts which permeate through the membrane means, wherein the membrane module means is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.
An apparatus for recovering bioproducts from a feed solution, said apparatus comprising:
- a housing having a feed inlet means, a feed outlet means, and a permeate outlet means;
- at least one membrane means disposed within the housing and having a feed side and a permeate side wherein a portion of the feed side of the membrane means is coated with a polymeric coating so as to render such portion impermeable to the bioproducts to be recovered;
- and
- a packed bed of adsorbent particles disposed within the housing in contact with the permeate side of the membrane;
- wherein the feed inlet means allows the feed solution to pass into contact with the feed side of the membrane;
- wherein bioproducts are separated from the feed solution by permeation to the permeate side of the membrane means;
- wherein the permeated bioproducts contact the packed bed;
- wherein the bioproducts are retained by the adsorbent particles;
- and
- whereby the bioproducts are isolated from the feed solution.
A method of recovering bioproducts from a feed solution, the method comprising:
- providing a membrane module comprising a housing, at least one membrane means disposed within the housing and having a feed side and a permeate side and wherein a portion of the feed side of the membrane means is coated with a polymeric coating so as to render such portion impermeable to the bioproducts to be recovered, and a packed bed of adsorbent particles disposed within the housing in contact with the permeate side of the membrane means;
- passing the feed solution into the membrane module to contact the feed side of the membrane;
- and
- separating bioproducts from the feed solution by permeation through to the permeate side of the membrane and allowing the permeated bioproducts to contact the adsorbent particles, wherein the bioproducts are retained by the adsorbent particles, whereby the bioproducts are isolated from the feed solution.
Dependencies are inferred from the claim text.