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AssigneeRongxiang Xu
InventorRongxiang Xu
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Compositions and methods are provided for culturing in vitro potentially regenerative cells (PRCs) from which functional tissue-organs are regenerated. In one aspect of the invention, a tissue culture medium is provided which comprises at least 50% of water and a sterol compound that is dissolved in a fatty acid-containing oil at a concentration at least 0.1% by weight based on the weight of the oil and added to the water. The culture medium can be used to culture PRCs that are isolated from the body of a mammal to generate functional tissue-organs in vitro with substantially the same physiological structure and function as the corresponding ones existing in vivo and in situ. The cultured PRCs, tissues, and tissue-organs can serve as valuable models for scientific investigation in life sciences, nutraceutical discovery, drug screening, pharmacokinetic studies, medical devices and tissue/organ transplantation.
A tissue culture medium, comprising:
A method for culturing potentially regenerative cells in vitro, comprising:
Isolated potentially regenerative cells from a predetermined site of the body of a mammal, wherein the isolated potentially regenerative cells, when cultured in a culture medium under suitable conditions, are capable of being activated to continuously proliferate and differentiate to form a tissue-organ which shares substantially the same physiological structure and at least one physiological function with that of the corresponding tissue in situ and in vivo.
Dependencies are inferred from the claim text.