Bacteriophages that infect bacillus bacteria (anthrax)
AssigneeUniversity of Northern Iowa Research Foundation
InventorMichael H. Walter
Life of this patent
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Abstract
The invention provides bacteriophages that infect Bacillus bacteria, including Bacillus anthracis, and compositions containing the bacteriophages. The invention also provides methods for using the bacteriophages of the invention to prevent and treat infection of an organism by Bacillus bacteria. Methods and materials to decontaminate a surface or an organism that is contaminated with Bacillus bacteria or Bacillus spores is also provided.
Claims57
An isolated bacteriophage selected from the group consisting of NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) and a recombinant form thereof.
An isolated bacteriophage containing genomic DNA that produces restriction digestion fragments that are within 10% of 18036, 14510, 11375, 9286, 4093, 2805, and 2054 basepairs when digested with restriction endonuclease Eco RI.
An isolated bacteriophage containing genomic DNA that produces restriction digestion fragments that are within 10% of 16801, 5679, 4690, 3057, and 1973 basepairs when digested with restriction endonuclease Eco RI.
An isolated bacteriophage containing genomic DNA that produces restriction digestion fragments that are within 10% of 19290, 16547, 11062, 8117, 8020 and 6245 basepairs when digested with restriction endonuclease Eco RI.
An antibacterial nutrient broth containing at least one bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) or a recombinant form thereof
A pharmaceutical composition comprising a bacteriophage selected from the group consisting of NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172) and MHWa (ATCC accession number PTA-4173), and a pharmaceutically acceptable carrier.
A method to decontaminate a surface that is contaminated by a Bacillus anthracis or a Bacillus anthracis spore comprising applying a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) or a recombinant form thereof to the surface.
A method to decontaminate an organism that has been contacted with Bacillus anthracis or with a Bacillus anthracis spore comprising applying a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) or a recombinant form thereof to the organism.
A method to treat a Bacillus anthracis infection in an organism comprising contacting the organism with a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) or a recombinant form thereof.
A method to prevent infection of an organism by Bacillus anthracis or an Bacillus anthracis spore comprising applying a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) or a recombinant form thereof to the organism.
A method to detect a bacterium or a bacterial spore in a sample comprising:
- a) contacting the sample with two or more bacteriophages that are incorporated into an electrical circuit, wherein binding of the bacterium or the bacterial spore to two or more bacteriophages changes an electrical characteristic, and
- b) detecting the changed electrical characteristic to indicate that a bacterium or a bacterial spore was present in the sample.
An apparatus for detecting a bacterium or a bacterial spore comprising:
- a) a mounting surface to which two or more bacteriophages are bound, wherein the bacteriophages can bind to the bacterium or the bacterial spore;
- b) means for providing an electrical signal to two or more bacteriophages, wherein contact of the bacterium or the bacterial spore with the two or more bacteriophages bound to the mounting surface changes an electrical characteristic;
- and
- c) a means for detecting the changed electrical characteristic.
A kit comprising a bacteriophage selected from NikoA (ATCC accession number PTA-4171), DDBa (ATCC accession number PTA-4172), MHWa (ATCC accession number PTA-4173) or a recombinant form thereof.
An antibody that can bind to a bacteriophage that can bind to a Bacillus bacterium.
A method to determine if a sample contains a bacterium or a bacterial spore comprising:
- a) contacting the sample with an antibody and a bacteriophage, wherein the bacteriophage binds to the bacterium or the bacterial spore and the antibody binds to the bacteriophage;
- b) treating the sample to separate unbound antibody from bound antibody;
- and
- c) detecting the presence of bound antibody to indicate the presence of a bacterium or bacterial spore in the sample.
An apparatus comprising:
- a) a liquid crystal;
- and
- b) a bacteriophage bound to the liquid crystal.
A method to determine whether or not a sample contains a bacterium or a bacterial spore comprising:
- a) contacting the sample with a liquid crystal to which a bacteriophage is bound;
- and
- b) determining whether or not a signal results from binding of the bacteriophage with a bacterium or bacterial spore.
A biosensor comprising a detector operatively coupled to a bacteriophage.
Dependencies are inferred from the claim text.