Production of biotin
AssigneeRoche Vitamins Inc.
InventorsTatsuo Hoshino, Akira Asakura, Tatsuya Kiyasu, Yoshie Nagahashi
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Abstract
The present invention provides processes for making biotin from desthiobiotin by either contacting desthiobiotin with an enzyme reaction mixture containing bioB gene product and nifU gene product and/or nifS gene product and isolating the biotin or cultivating a microorganism transformed with DNA encoding the bioB gene product, nifU gene product and nifS gene product and isolating the biotin.
Claims28
A process for making biotin by fermentation comprising cultivating, in an aqueous nutrient medium containing desthiobiotin, a microorganism transformed with a plasmid containing a DNA sequence encoding a bioB gene product and at least one additional DNA sequence selected from the group consisting of a DNA sequence encoding a nifU gene product and a DNA sequence encoding a nifS gene product, whereby the transformed microorganism expresses the gene products to convert desthiobiotin to biotin.
A process for making biotin by fermentation comprising cultivating, in an aqueous nutrient medium containing desthiobiotin, a microorganism transformed with a plasmid containing a DNA sequence encoding a bioB gene product and an additional plasmid containing at least one DNA sequence selected from the group consisting of a DNA sequence encoding a nifU gene product and a DNA sequence encoding a nifS gene product, whereby the transformed microorganism expresses the gene products to convert desthiobiotin to biotin.
Dependencies are inferred from the claim text.