US 2004/0063149 A1 · Method of identifying abnormal immune reactions

Abstract

A method for diagnosis of immunologically-mediated conditions such as allergic conditions, autoimmune diseases and immunological deficiency, and of assessing immunological reactivity associated with conditions such as viral infections, cancer, AIDS, tissue transplantation and the like. the method comprises: Taking a sample from a subject suspected to be manifesting an abnormal immunological reaction; isolating leukocytes from subject and a control; measuring the level of expression of a transcription factor in unstimulated leukocytes; stimulating the leukocytes by exposure to a stimulus; measuring the level of expression of a transcription factor in stimulated leukocytes; comparing results of stimulated and unstimulated leukocytes from subject and control; a significant difference in expression between the subject and control is indicative of an abnormal immunological reaction.

Claims25

Dependent claims

A method of assessing an immunological reaction in a test subject suspected to be manifesting an abnormal immunological reaction, comprising the steps of

substantially isolating leukocytes from a biological sample taken from a test subject and from a control subject;
stimulating said leukocytes by exposure to a stimulus;
measuring the level of expression of a transcription factor in said leukocytes;
and
comparing the levels of expression in stimulated leukocytes in the test subjects and in the control subjects and comparing the levels of expression to unstimulated leukocytes taken from these subjects,
wherein significantly different expression in the test subject than in the control subject is indicative of an abnormal immunological reaction.

3 more elements

Dependencies are inferred from the claim text.

Method of identifying abnormal immune reactions

Life of this patent

Method of identifying abnormal immune reactions

Life of this patent

Abstract

Claims25