Transcriptional regulator
AssigneesChugai Research Instititue for Molecular Medicine, Inc., a Japanese corporation, Chugai Seiyaku Kabushiki Kaisha, a Japan corporation
InventorMichael H. Jones
Life of this patent
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Abstract
Genes each encoding a novel transcriptional regulator having a bromodomain have been successfully isolated from a human testis cDNA library using primers prepared based on an EST sequence found using the bromodomain sequence of the transcriptional regulator. These genes are structurally analogous to each other.
Claims28
A substantially pure polypeptide comprising an amino acid sequence at least 60% identical to any one of SEQ ID NOs:
- 1, 13, 21, 27, or 29, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.
A substantially pure polypeptide comprising any one of SEQ ID NOs:
- 1, 13, 21, 27, or 29.
A substantially pure polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:
- 1, 13, 21, 27, or 29, with up to 30 conservative amino acid substitutions, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.
A substantially pure polypeptide encoded by a nucleic acid that hybridizes under high stringency conditions to a probe the sequence of which consists of any one of SEQ ID NOs:
- 2, 14, 22, 28, or 30, wherein the polypeptide regulates transcription of a gene and comprises a bromodomain.
An isolated nucleic acid comprising a strand that hybridizes under high stringency conditions to a single stranded probe consisting of any one of SEQ ID NOs:
- 2, 14, 22, 28, or 30.
A method of screening for a compound that binds to a polypeptide, the method comprising
- providing a polypeptide comprising an amino acid sequence at least 60% identical to SEQ ID NO:
- 1;
- contacting a test compound with the polypeptide;
- and
- determining whether the test compound has bound to the polypeptide.
Dependencies are inferred from the claim text.