Method of normalizing gene expression data

A method of normalizing gene expression data wherein, in a process for comparing expression quantities with respect to a plurality of genes, which expression quantities have been obtained for a first sample, and expression quantities with respect to the plurality of the genes, which expression quantities have been obtained for a second sample, with each other, data concerning the expression quantities having been obtained for the second sample are normalized, the method comprising the steps of:

• i) indicating the data concerning the expression quantities, which have been obtained for the first sample and the second sample, with points plotted on a logarithmic coordinate system, in which a horizontal axis represents logarithms of the expression quantities obtained for the first sample, and in which a vertical axis represents logarithms of the expression quantities obtained for the second sample,
• ii) calculating a coefficient from a value of an intercept of an approximate straight line, which is obtained from approximate representation of the plotted points with a straight line having a slope of 1, on the vertical axis, and
• iii) performing division processing for dividing the data concerning the expression quantities with respect to the plurality of the genes, which expression quantities have been obtained for the second sample, by the coefficient, whereby the data concerning the expression quantities having been obtained for the second sample are normalized.