Methods and apparatus for preparing and assaying biological samples to determine protein concentration
AssigneeProteoPlex, Inc.
InventorsSam LaBrie, Akash Mathur, Kim Lawry, Heather Pancio, Nathan Danielson
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Abstract
The present invention discloses methods and apparatus for the detection of biological molecules in various samples. In particular, a method for detecting a hapten-labeled analyte in a solution wherein substantially all other solution constituents are labeled with a hapten is disclosed. The method may be used to detect single or multiple analytes in solution. Apparatus to detect such analytes are also disclosed.
Claims75
A method for detecting an analyte molecule in a solution, said analyte molecule and substantially all other solution constituents being labeled with a hapten, comprising the step of contacting the solution with a population of only one species of capture agent, said capture agent having affinity for said analyte molecule, wherein the analyte molecule is thereby detected.
A method for quantitating an analyte molecule in a solution, said analyte molecule and substantially all other solution constituents being labeled with a hapten, comprising the step of contacting the solution with a population of only one species of capture agent, said capture agent having affinity for said analyte molecule, and measuring the quantity of captured analyte molecules.
A method for quantitating at least two different analytes in solution, said analytes and substantially all other solution constituents being labeled with a hapten, comprising:
- (a) contacting the solution with at least two different species of capture agents, each of said capture agents having affinity toward a different analyte;
- and
- (b) measuring the quantity of captured analytes.
A method for quantitating at least two different analytes in a solution comprising the steps of:
- (a) attaching to a support at least two different species of capture agents, each of said capture agents having affinity toward a different analyte;
- (b) labeling substantially all of the analytes in the solution with a hapten;
- (c) contacting the solution with the capture agents;
- and
- (d) measuring the quantity of captured analytes.
A method for quantitating at least two different analytes in a solution comprising the steps of:
- (a) attaching to a support at least two different species of capture agents, each of said capture agents having affinity toward a different analyte;
- (b) mixing the solution with an excess amount of hapten such that substantially all of the solution constituents are bound with hapten;
- (c) saturating unbound hapten from step (b) such that the unbound hapten is rendered substantially inactive;
- (d) contacting the solution of steps (b) and (c) with the capture agents of step (a);
- (e) adding to the solution an excess amount of at least one hapten binding partner exhibiting high affinity toward the hapten, said hapten binding partner being attached to a detection probe;
- (f) removing unbound hapten binding partner from the biological sample;
- and
- (g) measuring the quantity of captured analytes.
A method for quantitating at least two different analytes in a solution using a microarray platform comprising the steps of:
- (a) attaching at least two different species of capture agents to the microarray platform, said microarray platform comprising a multi-well tray, such that each well of the multi-well tray contains an ordered matrix of species of capture agents;
- (b) labeling substantially all of the analytes in the solution with hapten;
- (c) contacting the solution of step (b) with each binding site;
- and
- (d) measuring the quantity of captured analytes.
A microarray platform comprising different species of capture agents exhibiting affinity toward different analyte molecules in a solution, wherein substantially all solution constituents are labeled with a hapten, and wherein the solution is in contact with at least one capture agent.
A kit for detecting the concentration of an analyte in a solution comprising at least one control analyte, a hapten, a hapten binding partner, a detection probe, and reagents, wherein said reagents facilitate the binding of the detection probe to the analyte in a solution.
Dependencies are inferred from the claim text.