Cell adhesion resisting surfaces
OwnerAndrea Liebmann-Vinson, Richard P. Clarke +2inventors
InventorsAndrea Liebmann-Vinson, Richard P. Clarke, Ruiling Xu, Mohammad A. Heidaran
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Abstract
A coated surface that resists cell adhesion comprisesg hyaluronic acid directly bound to a plasma-treated polymer surface. A process for producing the coated surface is disclosed as are further modifications of the hyaluronic acid by attaching ligand-binding polypeptides (antibodies or antibody binding proteins).
Claims60
A process of coating a surface of an article with hyaluronic acid (HA) or alginic acid (AA) or a derivative of HA or AA, which consists essentially of the steps of
- i) treating a surface of an article with a plasma that causes formation of nitrogen containing groups on said surface;
- and
- ii) exposing the treated surface of the article to a solution containing hyaluronic acid or alginic acid, or a derivative thereof, in the presence of a carbodiimide and a reactive intermediate ester stabilizing compound;
- to obtain a coated surface.
A process of coating a plasma-treated surface of an article, which surface comprises nitrogen-containing groups, with HA or AA or a derivative of HA or AA, said process comprising:
- exposing the plasma-treated surface to a solution of HA, AA or said derivative in the presence of a carbodiimide and a reactive intermediate stabilizing compound, to obtain a coated surface.
A process of coating a plasma-treated surface of an article, which surface comprises nitrogen-containing groups, with HA or AA or a derivative of HA or AA, said process consisting essentially of:
- exposing the plasma-treated surface to a solution of HA, AA or said derivative in the presence of a carbodiimide and a reactive intermediate stabilizing compound, to obtain a coated surface.
An article useful for binding a target cell or target molecule, which target cell or molecule comprises a target ligand, said article comprising;
- (a) a CAR surface coated with a layer comprising HA, AA, or a derivative of HA or AA;
- (b) covalently bonded to said HA, AA or derivative, a first ligand-binding polypeptide that is capable of binding to said target ligand.
51 The article of claim 48, further comprising, bound to said covalently bonded first ligand binding polypeptide,
- (c) a second ligand binding polypeptide that:
- (i) comprises a target ligand for said first ligand binding polypeptide, and
- (ii) is capable of binding to a target ligand of a target cell or target molecule.
The article of claim 51 wherein said second ligand-binding polypeptide is (a) an antibody or antigen-binding fragment thereof, (b) and immunoglobulin-binding protein (c) a receptor, (d) a lectin, (e) a cell adhesion molecule, (f) an extracellular matrix peptide or polypeptide or (f) a synthetic peptide.
The article of claim 51 wherein said second ligand-binding polypeptide is a anti-CD34 monoclonal antibody or an antigen binding fragment or derivative thereof.
Dependencies are inferred from the claim text.