True breeding transgenics from plants heterozygous for transgene insertions
AssigneeNew Zealand Institute for Crop & Food Research Lim
InventorAnthony John Conner
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Abstract
The invention provides a method for biasing a crop plant which is heterozygous for a transgene towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene coding for resistance to a specific phytotoxin with the specific phytotoxin one or more times during the life of the crop plant. The method achieves this through selective inhibition of phytotoxin-sensitive plant ovules, embryos and pollen. The method has particular application to the production of open pollinated and synthetic varieties of crop plants, such as alfalfa synthetic varieties.
Claims21
A method for biasing a crop plant which is heterozygous for a transgene coding for resistance to a specific phytotoxin towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene with the specific phytotoxin one or more times during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety.
A method of selectively inhibiting phytotoxin-sensitive plant ovules, embryos and/or pollen in order to bias a crop plant which is heterozygous for a transgene coding for resistance to a specific phytotoxin towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene with the specific phytotoxin one or more times during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety.
A method of selectively inhibiting phytotoxin-sensitive plant ovules in order to bias a crop plant which is heterozygous for a transgene coding for resistance to a specific phytotoxin towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene with the specific phytotoxin one or more times during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety.
A method of selectively aborting phytotoxin-sensitive plant embryos in order to bias a crop plant which is heterozygous for a transgene coding for resistance to a specific phytotoxin towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene with the specific phytotoxin one or more times during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety.
A method of selectively inhibiting phytotoxin-sensitive pollen in order to bias a crop plant which is heterozygous for a transgene coding for resistance to a specific phytotoxin towards the production of seeds which carry the transgene comprising the step of contacting the crop plant containing a gene construct comprising the transgene with the specific phytotoxin one or more times during the life of the crop plant, wherein the crop plant is part of an open-pollinated population or a synthetic variety.
The method of claim 12 wherein the herbicide is chlorsulfuron.
A method of producing a transgenic synthetic alfalfa variety comprising the steps of:
- 1) producing a transgenic alfalfa plant, wherein the transgenic alfalfa plant comprises an introduced gene construct which comprises a heterologous gene coding for phytotoxin resistance operably linked to a heterologous gene of interest;
- 2) using the transgenic alfalfa plant produced by step 1) as a pollen parent and crossing the transgenic alfalfa plant to one or more alfalfa plants which do not contain the phytotoxin gene operably linked to the heterologous gene of interest;
- 3) applying the phytotoxin one or more times during the life cycle of the plants in step 2);
- and,
- 4) harvesting and bulking the resultant seed to produce a new synthetic alfalfa variety which contains the phytotoxin gene operably linked to the gene of interest.
Dependencies are inferred from the claim text.