Multimerized DBH enhancer domains
AssigneeThe McLean Hospital Corporation
InventorKwang-Soo Kim
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Abstract
The invention features an enhancer cassette having the formula [X-Y]n, wherein each X is independently a noradrenergic cell-specific enhancer derived from a DBH gene; Y is absent or is a mono or polynucleotide that has between one and thirty nucleotides; and n is an integer between three and twenty, inclusive.
Claims17
An enhancer cassette having the formula [X-Y]n, wherein each X is, independently, selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 39, SEQ ID NO:
- 40, and SEQ ID NO:
- 41;
- each Y is, independently, absent or a mono- or polynucleotide that contains between one and thirty nucleotides;
- and n is an integer between two and fifty, inclusive.
An expression vector comprising an enhancer cassette having the formula [X-Y]n, wherein each X is independently selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 39, SEQ ID NO:
- 40, and SEQ ID NO:
- 41;
- each Y is, independently, absent or a mono or polynucleotide that has between one and thirty nucleotides;
- and n is an integer between one and fifty, inclusive.
A method of expressing a nucleic acid molecule in a noradrenergic cell, comprising expressing in said noradrenergic cell an expression vector comprising an enhancer cassette having the formula [X-Y]n, operably linked to said nucleic acid molecule, wherein each X is independently selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 8, SEQ ID NO:
- 39, SEQ ID NO:
- 40, and SEQ ID NO:
- 41;
- each Y is, independently, absent or a mono or polynucleotide that has between one and thirty nucleotides;
- and n is an integer between three and fifty, inclusive.
A substantially pure nucleic acid molecule consisting essentially of SEQ ID NO:
- 6.
A substantially pure nucleic acid molecule consisting essentially of SEQ ID NO:
- 8.
A substantially pure nucleic acid molecule consisting essentially of SEQ ID NO:
- 39
A substantially pure nucleic acid molecule consisting essentially of SEQ ID NO:
- 40.
A substantially pure nucleic acid molecule consisting essentially of SEQ ID NO:
- 41.
An enhancer cassette comprising a polynucleotide having the formula X-Y-X-Y-X, wherein each X is, independently, selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 39 and SEQ ID NO:
- 41 and each Y is, independently, absent or a mono or polynucleotide that has between one and fifty nucleotides, inclusive, wherein SEQ ID NO:
- 1, SEQ ID NO:
- 39 and SEQ ID NO:
- 41 are present in said enhancer cassette.
An expression vector comprising an enhancer cassette having the formula X-Y-X-Y-X, wherein each X is, independently, selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 39 and SEQ ID NO:
- 41 and each Y is, independently, absent or a mono or polynucleotide that has between one and fifty nucleotides, inclusive, wherein SEQ ID NO:
- 1, SEQ ID NO:
- 39 and SEQ ID NO:
- 41 are present in said enhancer cassette.
A method of expressing a nucleic acid molecule in a noradrenergic cell, comprising expressing in said noradrenergic cell an expression vector comprising an enhancer cassette having the formula X-Y-X-Y-X operably linked to said nucleic acid molecule, wherein each X is, independently, selected from the group consisting of SEQ ID NO:
- 1, SEQ ID NO:
- 39 and SEQ ID NO:
- 41 and each Y is, independently, absent or a mono or polynucleotide that has between one and fifty nucleotides, inclusive, wherein SEQ ID NO:
- 1, SEQ ID NO:
- 39 and SEQ ID NO:
- 41 are present in said enhancer cassette.
Dependencies are inferred from the claim text.