Infection model

Method for preparing a monolayer human or animal in vitro tissue test system according to one of claims 1 to 5, comprising the extraction of collagen-containing tissue, the transfer of the collagen-containing tissue into acidic solution, the incubation of the collagen tissue transferred into acidic solution at 2 to 10° C., in particular at 4° C., the centrifuging off of undissolved collagen parts, the mixing of the obtained collagen solution at 2 to 10° C., preferably at 4° C., with a solution containing the human or animal cells to be cultivated, cell culture medium, serum, and buffer, and the gelling of the mixed solution by increasing the temperature.

Method for preparing a three-dimensional, multilayer human or animal in vitro organ or tissue test system, wherein cells of a first human or animal tissue type, in a three-dimensional, gel-like biomatrix containing the cells to be cultivated and at least 3 mg/ml collagen in buffered, serum-containing cell culture medium, wherein the collagen solution used for preparing the biomatrix has a content of non-denatured, native collagen of ≧90% in acidic, aqueous medium, undergo at least a one to two day cultivation, and wherein cells of a second human or animal tissue type are then seeded in a cell culture medium on the biomatrix and are then further cultivated, and wherein, in an optional further step, cells of further human or animal tissue types are then potentially seeded in a cell culture medium and are then further cultivated so that a three-dimensional, multilayer in vitro organ or tissue test system is obtained.