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US 2004/0023401 A1Published application
Detection method of proteins on polyacrylamide gels using a dye composition as a silver ion sensitizing agent and the dye composition for the same
AssigneesSan-Ock JEONG, EZ BioPaQ Co., Ltd.
InventorJung-Kap Choi
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Abstract
The present invention relates to a method for detecting proteins on polyacrylamide gels, comprising the steps of sensitizing the proteins with a dye composition comprising an acidic organic dye as a silver ion sensitizing agent, and optionally, a basic organic dye as an auxiliary agent, and washing them, and then silver staining complexes of the protein-dye, and to the dye composition for the same.
Claims9
A method for detecting proteins on polyacrylamide gels, comprising the steps of:
- (a) sensitizing the proteins with a dye composition containing one or more acidic organic dyes selected from the group consisting of calcon dye derivatives, alizarin yellow GG, alizarin yellow R, zincon, Bordeaux R, tryphan blue, and acid blue 92 as a silver ion sensitizing agent, and then, washing them;
- (b) impregnating silver ions to complexes of the protein-dye, and then, reducing them to metallic silver;
- and
- (c) observing the protein-dye-metallic silver complexes.
1 more element
Dependencies are inferred from the claim text.