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AssigneeBoard of Control of Michigan Technological University
InventorsGopi Krishna Podila, Leland James Cseke, Banalata Sen, David F. Karnosky
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The present invention provides compositions and methods for producing a transgenic plant that exhibits altered characteristics resulting from over expression or under expression of a novel polypeptide PtM3 or its homolog PtM4. The altered characteristics resulting from over-expression include at least one of the ability to convert axillary meristem to floral meristem; to accelerate flowering i.e., early flowering; to increase fruit production; to increase nut production; to increase seed output; to increase branching; to increase flower production; to increase fruit yield; to increase flower yield and a combination thereof. The altered characteristics resulting from suppressed expression include at least one of complete sterility; partial sterility (sterility of only one sex of a bisexual plant); reduced pollen production; decreased flowering; increased biomass and combinations thereof. Furthermore, once the transgenic plant is sterile, additional exogenous sequences may be incorporated into the sterile plant genome, resulting in other desired plant characteristics. Related promoter, gene constructs, methods, antibodies and kits are also provided.
An isolated polynucleotide encoding a polypeptide, wherein the polypeptide is a PtM3 polypeptide.
An isolated polynucleotide encoding a polypeptide, wherein the polypeptide is a PtM4 polypeptide.
An isolated polynucleotide comprising a nucleotide sequence that hybridizes under highly stringent conditions to a hybridization probe, the nucleotide sequence which comprises:
An isolated polynucleotide comprising a nucleotide sequence that is a degenerate variant of:
An isolated polynucleotide comprising a nucleotide sequence that encodes a polypeptide with the amino acid sequence which comprises at least one of
A gene construct comprising:
A gene construct comprising:
A method of producing a transgenic plant having at least one altered characteristic compared to a nontransgenic control plant, wherein the method comprises:
A method of producing a transgenic plant having an altered characteristic compared to a nontransgenic control plant, the method comprising:
A method of altering the reproductive capacity of a plant comprising incorporating into the genome of the plant, a recombinant polynucleotide molecule comprising a regulatory element having a MAD-box motif, operably linked to a polynucleotide molecule encoding the polypeptide PtM3 or PtM4, such that when the polynucleotide molecule is overexpressed in the plant, the reproductive capacity of the plant is altered, resulting in a characteristic including at least one of increased flowering time, increased flowering numbers, increased branching, conversion of axillary meristems to floral meristems, increased fruit production, increased nut production, increased seed output, early reproductive development, and combinations thereof compared to a control plant that is not transformed with the nucleotide sequence.
A method of altering the reproductive capacity of a plant comprising incorporating into the genome of the plant, a recombinant polynucleotide molecule comprising a regulatory element having a MAD-box motif, operably linked to a polynucleotide molecule encoding PtM3 or PtM4, such that when the polynucleotide sequence is expressed in the plant, a characteristic of the plant is altered, wherein the characteristic is selective inhibition of reproductive structures or reproductive sterility as compared to a control plant that is not transformed with the nucleotide sequence.
A plant having a characteristic genetically altered through incorporation into the genome of the plant including at least one of a nucleotide sequence which comprises a promoter sequence of PtM3, a functionally equivalent PtM3 promoter fragment thereof, a nucleotide sequence encoding a polypeptide PtM3, or a functionally equivalent PtM3 amino acid fragment thereof, and a combination thereof, the altered characteristic including at least one of (i) converting axillary meristem to floral meristem;
A tree having a characteristic genetically altered through incorporation into the genome of the tree including at least one of a nucleotide sequence which comprises a promoter sequence of PtM3, a functionally equivalent PtM3 promoter fragment thereof, a nucleotide sequence encoding a polypeptide PtM3, or a functionally equivalent PtM3 amino acid fragment thereof, and a combination thereof, the altered characteristic including at least one of (i) converting axillary meristem to floral meristem;
A plant having a characteristic genetically altered through incorporation into the genome of the tree including at least one of a promoter sequence of PtM3, a functionally equivalent PtM3 promoter fragment thereof, a nucleotide sequence encoding a polypeptide PtM3, or a functionally equivalent PtM3 amino acid fragment thereof, and a combination thereof, the altered characteristic comprising sterility of at least one of the reproductive structures compared to a control plant that is not transformed with the nucleotide sequence.
A tree having a characteristic genetically altered through incorporation into the genome of the tree including at least one of a nucleotide sequence which comprises a promoter sequence of PtM3, a functionally equivalent PtM3 promoter fragment thereof, a nucleotide sequence encoding a polypeptide PtM3, or a functionally equivalent PtM3 amino acid fragment thereof, and a combination thereof, the altered characteristic comprises sterility of at least one of the reproductive structures compared to a control tree that is not transformed with the nucleotide sequence.
An isolated polynucleotide comprising a nucleotide sequence as shown in SEQ ID NO:3, or a degenerate variant of SEQ ID NO:3.
A polynucleotide comprising a nucleotide sequence that hybridizes under highly stringent conditions to a hybridization probe, the nucleotide sequence of which comprises of SEQ ID NO:3, or a complement, a reverse complement, and a reverse sequence of SEQ ID NO:3.
An isolated and purified polynucleotide molecule comprising a polynucleotide sequence of SEQ ID NO:3 or a transcriptional regulatory region thereof, wherein when the polynucleotide sequence is operatively linked with a nucleotide sequence encoding a RNAse, the polynucleotide transcriptional regulatory region is degraded, resulting in a sterile plant.
A gene promoter, comprising:
A gene promoter, comprising:
A purified polypeptide sequence comprising the amino acid sequence of
A method of eliciting an immune response in an animal, comprising introducing into the animal a composition comprising
A method of generating antibodies comprising introducing into an animal a composition comprising an amino acid sequence specific for at least one of
A purified antibody comprising an amino acid sequence that binds specifically to at least one of
A kit for producing a transgenic plant characterized by having at least one of (i) conversion of axillary meristem to floral meristem;
A kit for producing a transgenic plant, wherein the transgenic plant includes at least one of a completely sterile plant, a partially sterile plant and combinations thereof, wherein the kit includes at least one of a PtM3 polypeptide having at least 75% amino acid identity with SEQ ID NO:4 or SEQ ID NO:5, and a promoter to facilitate polypeptide expression.
Dependencies are inferred from the claim text.