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AssigneeAtheroGenics, Inc.
InventorsAnne M. Whalen, Christopher K. Cook, James A. Sikorski
Estimating the term…
Isolated nucleic acid molecules encoding human MEKK2, and isolated human MEKK2 proteins, are provided. The invention further provides antisense nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals carrying a human MEKK2 transgene. The invention further provides human MEKK2 fusion proteins and anti-human MEKK2 antibodies. Methods of using the human MEKK2 proteins and nucleic acid molecules of the invention are also disclosed, including methods for detecting human MEKK2 activity in a biological sample, methods of modulating human MEKK2 activity in a cell, and methods for identifying agents that modulate the activity of human MEKK2.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding a MEKK2 protein, said MEKK2 protein having an amino acid sequence at least 99.1% identical to the amino acid sequence of SEQ ID NO:2, wherein % identity is determined according to the LALIGN algorithm using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.
An isolated nucleic acid molecule which encodes a MEKK2 protein comprising the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid molecule which comprises the nucleotide sequence of SEQ ID NO:3.
An isolated nucleic acid molecule encoding a MEKK2 protein comprising amino acids 356-616 of SEQ ID NO:2.
An isolated nucleic acid molecule encoding a MEKK2 fragment consisting essentially of amino acids 356-616 of SEQ ID NO:2.
An isolated nucleic acid molecule encoding a fusion protein comprising a MEKK2 fragment consisting essentially of amino acids 356-616 of SEQ ID NO:2 and at least one non-MEKK2 polypeptide.
An isolated MEKK2 protein comprising an amino acid sequence at least 99.1% identical to the amino acid sequence of SEQ ID NO:2, wherein % identity is determined according to the LALIGN algorithm using a PAM120 weight residue table, a gap length penalty of 12 and a gap penalty of 4.
An isolated protein comprising the amino acid sequence of SEQ ID NO:2.
An isolated protein encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO3.
An isolated protein comprising amino acids 356-616 of SEQ ID NO:2.
An isolated MEKK2 fragment consisting essentially of amino acids 356-616 of SEQ ID NO:2.
A fusion protein comprising a MEKK2 fragment consisting essentially of amino acids 356-616 of SEQ ID NO:2 and at least one non-MEKK2 polypeptide.
A nonhuman transgenic animal that having the gene encoding the MEKK2 protein set forth as SEQ ID NO:2 mutated or deleted such that the animal fails to express the MEKK2 protein.
A method for identifying a MEKK2-specific modulator comprising:
Use of the MEKK2 protein set forth as SEQ ID NO:2 in a method for identifying a compound which specifically modulates the expression or activity of a non-MEKK2 protein, wherein a lack of expression or activity of said MEKK2 protein as compared to the expression or activity of the non-MEKK2 protein indicates that the compound specifically modulates the expression or activity of the non-MEKK2 protein.
Dependencies are inferred from the claim text.