US 2004/0018976 A1 · Polynucleotide encoding novel human G-protein coupled receptors, and splice variants thereof

Abstract

The present invention provides novel polynucleotides encoding HGPRBMY30_1, HGPRBMY30_2, HGPRBMY30_3, HGPRBMY41_1, HGPRBMY41_2, HGPRBMY41_3, HGPRBMY42, HGPRBMY42_1, HGPRBMY43, and/or HGPRBMY44 polypeptides, fragments and homologues thereof. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel HGPRBMY30_1, HGPRBMY30_2, HGPRBMY30_3, HGPRBMY41_1, HGPRBMY41_2 HGPRBMY41_3, HGPRBMY42, HGPRBMY42_1, HGPRBMY43, and/or HGPRBMY44 polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:2 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:1, having biological activity;
(f) an isolated polynucleotide comprising nucleotides 4 to 2589 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 863 of SEQ ID NO:2 of SEQ ID NO:2 minus the start methionine;
(g) an isolated polynucleotide comprising nucleotides 1 to 2589 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 863 of SEQ ID NO:2 including the start methionine;
(h) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:1;
(i) a polynucleotide fragment of SEQ ID NO:3 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3;
(j) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:4 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3;
(k) a polynucleotide encoding a polypeptide domain of SEQ ID NO:4 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3;
(l) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:4 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3;
(m) a polynucleotide encoding a polypeptide of SEQ ID NO:4 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:3, having biological activity;
(n) an isolated polynucleotide comprising nucleotides 4 to 2487 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 829 of SEQ ID NO:4 of SEQ ID NO:4 minus the start methionine;
(o) an isolated polynucleotide comprising nucleotides 1 to 2487 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 829 of SEQ ID NO:4 including the start methionine;
(p) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:3;
(q) a polynucleotide fragment of SEQ ID NO:5 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5;
(r) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:6 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5;
(s) a polynucleotide encoding a polypeptide domain of SEQ ID NO:6 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5;
(t) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:6 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5;
(u) a polynucleotide encoding a polypeptide of SEQ ID NO:6 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:5, having biological activity;
(v) an isolated polynucleotide comprising nucleotides 4 to 2682 of SEQ ID NO:5, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 894 of SEQ ID NO:6 of SEQ ID NO:6 minus the start methionine;
(w) an isolated polynucleotide comprising nucleotides 1 to 2682 of SEQ ID NO:5, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 894 of SEQ ID NO:6 including the start methionine;
(x) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:5;
(y) a polynucleotide fragment of SEQ ID NO:7 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:7;
(z) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:8 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:7;
(aa) a polynucleotide encoding a polypeptide domain of SEQ ID NO:8 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:7;
(bb) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:8 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:7;
(cc) a polynucleotide encoding a polypeptide of SEQ ID NO:8 or the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:7, having biological activity;
(dd) an isolated polynucleotide comprising nucleotides 4 to 1200 of SEQ ID NO:7, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 400 of SEQ ID NO:8 of SEQ ID NO:8 minus the start methionine;
(ee) an isolated polynucleotide comprising nucleotides 1 to 1200 of SEQ ID NO:7, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 400 of SEQ ID NO:8 including the start methionine;
(ff) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:7;
(gg) a polynucleotide fragment of SEQ ID NO:9 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:9;
(hh) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:10 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:9;
(ii) a polynucleotide encoding a polypeptide domain of SEQ ID NO:10 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:9;
(jj) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:10 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:9;
(kk) a polynucleotide encoding a polypeptide of SEQ ID NO:10 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:9, having biological activity;
(ll) an isolated polynucleotide comprising nucleotides 4 to 1647 of SEQ ID NO:9, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 549 of SEQ ID NO:10 of SEQ ID NO:10 minus the start methionine;
(mm) an isolated polynucleotide comprising nucleotides 1 to 1647 of SEQ ID NO:9, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 549 of SEQ ID NO:10 including the start methionine;
(nn) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:9;
(oo) a polynucleotide fragment of SEQ ID NO:11 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:11;
(pp) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:12 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:11;
(qq) a polynucleotide encoding a polypeptide domain of SEQ ID NO:12 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:11;
(rr) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:12 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:11;
(ss) a polynucleotide encoding a polypeptide of SEQ ID NO:12 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:11, having biological activity;
(tt) an isolated polynucleotide comprising nucleotides 4 to 1365 of SEQ ID NO:11, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 455 of SEQ ID NO:12 of SEQ ID NO:12 minus the start methionine;
(uu) an isolated polynucleotide comprising nucleotides 1 to 1365 of SEQ ID NO:11, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 455 of SEQ ID NO:12 including the start methionine;
(vv) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:11;
(ww) a polynucleotide fragment of SEQ ID NO:13 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:13;
(xx) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:14 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:13;
(yy) a polynucleotide encoding a polypeptide domain of SEQ ID NO:14 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:13;
(zz) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:14 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:13;
(aaa) a polynucleotide encoding a polypeptide of SEQ ID NO:14 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:13, having biological activity;
(bbb) an isolated polynucleotide comprising nucleotides 4 to 1524 of SEQ ID NO:13, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 508 of SEQ ID NO:14 of SEQ ID NO:14 minus the start methionine;
(ccc) an isolated polynucleotide comprising nucleotides 1 to 1524 of SEQ ID NO:13, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 508 of SEQ ID NO:14 including the start methionine;
(ddd) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:13;
(eee) a polynucleotide fragment of SEQ ID NO:15 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:15;
(fff) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:16 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:15;
(ggg) a polynucleotide encoding a polypeptide domain of SEQ ID NO:16 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:15;
(hhh) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:16 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:15;
(iii) a polynucleotide encoding a polypeptide of SEQ ID NO:16 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:15, having biological activity;
(jjj) an isolated polynucleotide comprising nucleotides 4 to 1194 of SEQ ID NO:15, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 398 of SEQ ID NO:16 of SEQ ID NO:16 minus the start methionine;
(kkk) an isolated polynucleotide comprising nucleotides 1 to 1194 of SEQ ID NO:15, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 398 of SEQ ID NO:16 including the start methionine;
(lll) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:15;
(mmm) a polynucleotide fragment of SEQ ID NO:17 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:17;
(nnn) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:18 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:17;
(ooo) a polynucleotide encoding a polypeptide domain of SEQ ID NO:18 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:17;
(ppp) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:18 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:17;
(qqq) a polynucleotide encoding a polypeptide of SEQ ID NO:18 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:17, having biological activity;
(rrr) an isolated polynucleotide comprising nucleotides 4 to 1167 of SEQ ID NO:17, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 389 of SEQ ID NO:18 of SEQ ID NO:18 minus the start methionine;
(sss) an isolated polynucleotide comprising nucleotides 1 to 1167 of SEQ ID NO:17, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 389 of SEQ ID NO:18 including the start methionine;
(ttt) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:17;
(uuu) a polynucleotide fragment of SEQ ID NO:19 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:19;
(vvv) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:20 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:19;
(www) a polynucleotide encoding a polypeptide domain of SEQ ID NO:20 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:19;
(xxx) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:20 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:19;
(yyy) a polynucleotide encoding a polypeptide of SEQ ID NO:20 or the cDNA sequence included in ATCC Deposit No:
XXXXX, which is hybridizable to SEQ ID NO:19, having biological activity;
(zzz) an isolated polynucleotide comprising nucleotides 4 to 2778 of SEQ ID NO:19, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 926 of SEQ ID NO:20 of SEQ ID NO:20 minus the start methionine;
(aaaa) an isolated polynucleotide comprising nucleotides 1 to 2778 of SEQ ID NO:19, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 926 of SEQ ID NO:20 including the start methionine;
(bbbb) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:19;
and
(cccc) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(bbbb), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

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Polynucleotide encoding novel human G-protein coupled receptors, and splice variants thereof

Life of this patent

Polynucleotide encoding novel human G-protein coupled receptors, and splice variants thereof

Life of this patent

Abstract

Claims20