Modified CDC14 phosphatase proteins and constructs for improved expression and purification
AssigneeApplera Corporation
InventorsCraig Hill, Joseph Buggy
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Abstract
The present invention provides modified Cdc14 phosphatase proteins, and novel constructs for expression thereof, in which the sequence C-terminal to the catalytic domain has been deleted. This modification results in the expression of a single form of the enzyme, enabling purification of a homogeneous preparation of active enzyme at a high yield.
Claims25
An isolated polypeptide consisting of an amino acid sequence selected from the group consisting of:
- (a) SEQ ID NO:1;
- and
- (b) SEQ ID NO:2.
An isolated polypeptide consisting of an amino acid sequence selected from the group consisting of:
- (a) an amino acid sequence having at least 99% sequence identity to SEQ ID NO:1;
- and
- (b) an amino acid sequence having at least 99% sequence identity to SEQ ID NO:2.
An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence that encodes an amino acid sequence consisting of SEQ ID NO:1;
- (b) a nucleotide sequence that encodes an amino acid sequence consisting of SEQ ID NO:2;
- (c) SEQ ID NO:3;
- and
- (d) a nucleotide sequence that is completely complementary to a nucleotide sequence of (a)-(c).
An isolated nucleic acid molecule consisting of a nucleotide sequence selected from the group consisting of:
- (a) a nucleotide sequence that encodes a polypeptide consisting of an amino acid sequence having at least 99% sequence identity to SEQ ID NO:1;
- (b) a nucleotide sequence that encodes a polypeptide consisting of an amino acid sequence having at least 99% sequence identity to SEQ ID NO:2;
- (c) a nucleotide sequence having at least 99% sequence identity to SEQ ID NO:3;
- and
- (d) a nucleotide sequence that is completely complementary to a nucleotide sequence of (a)-(c).
An isolated polypeptide consisting of the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide consisting of the nucleotide sequence of SEQ ID NO:3.
Dependencies are inferred from the claim text.