Process for producing polypeptide having disulfide bond

Abstract

The present invention relates to a DNA which enables co-expression of a protein disulfide isomerase and a polypeptide having disulfide bonds, comprising one or two identical or different promoters required for gene expression (Promoter), two identical or different Shine-Dalgarno sequences (SD), two identical or different sequences encoding signal peptides of cell wall proteins (CWP) of a bacterium of the genus Bacillus (CWPsp), a gene encoding a polypeptide having disulfide bonds (Polypeptide), and a gene encoding protein disulfide isomerase (PDI) that are ligated to each other as represented by the following formula: 5′-Promoter-SD-CWPsp-X1-(Promoter)n-SD-CWPsp-X2-3′, wherein X1=Polypeptide and X2=PDI, or X1=PDI and X2=Polypeptide, and n=0 or 1. The present invention relates to also a process for producing polypeptides using the DNA. The present invention can enhance the efficiency of formation of correct disulfide bond in an expression system of a gene recombinant polypeptide having disulfide bonds.

Dependencies are inferred from the claim text.