Methods and compositions for protein expression and purification
AssigneeLifesensors, Inc.
InventorsTauseef R. Butt, Stephen D. Weeks, Hiep T. Tran, Michael P. Malakhov, Oxana A. Malakhova
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Abstract
Methods for enhancing expression levels and secretion of heterologous fusion proteins in a host cell are disclosed.
Claims10
A kit comprising a recombinant vector containing a nucleic acid sequence encoding a UBL molecule selected from the group of SUMO, RUB, HUB, URM1, and ISG15 operably linked to a promoter suitable for expression in the desired host cell and a multiple cloning site suitable for cloning a nucleic acid encoding the protein of interest in-frame with the nucleic acid sequence encoding the UBL molecule.
A kit for purification of a protein from a host cell comprising:
- i) a recombinant vector containing a nucleic acid sequence encoding a UBL molecule selected from the group of SUMO, RUB, HUB, URM1, and ISG15 operably linked to a promoter suitable for expression in the desired host cell, a nucleic acid sequence encoding for a purification tag in-frame with the nucleic acid sequence encoding the UBL molecule, and a multiple cloning site suitable for cloning a nucleic acid encoding the protein of interest in-frame with the nucleic acid sequence encoding the UBL molecule, and
- ii) a protease composition capable of cleaving the UBL molecule from the fusion protein.
Dependencies are inferred from the claim text.