Device and method for purification of nucleic acids

A method of manufacturing a device, comprising:

• providing ion-exchange particles;
• providing a support including at least one protrusion extending therefrom;
• and
• contacting the protrusion with the ion-exchange particles such that the ion-exchange particles are affixed to the protrusion.
• contacting the ion-exchange particles with a size-exclusion resin to at least one of encapsulate and micro-encapsulate the ion-exchange particles.

A method of manufacturing a purification device, comprising:

• providing a mold;
• disposing ion-exchange particles in the mold;
• disposing reactive monomer solution to the mold;
• and
• reacting the monomer solution to form a size-exclusion resin that embeds the ion-exchange particles.

A method comprising:

• providing a purification device comprising a substrate comprising ion-exchange material and size exclusion resin, wherein the ion-exchange material at least partially contacts the size-exclusion resin;
• providing a sample solution;
• and
• contacting the purification device with the sample solution for a period of time sufficient to remove impurities from the sample solution and form a purified sample solution.

A kit for purification of a sample solution, wherein the kit comprises:

• ion-exchange particles;
• a reactive monomer composition capable of forming a size-exclusion resin;
• and
• a receptacle capable of receiving the ion-exchange particles and the reactive monomer composition.