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US 2004/0006795 A1Published application
Methods to increase the isoflavonoid levels in plants and plants producing increased levels of isoflavonoids
AssigneeE. I. Du Pont de Nemours and Company
InventorsBrian McGonigle, Joan T. Odell
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Abstract
This invention pertains to methods of increasing isoflavonoid production in isoflavonoid-producing plants by transforming plants with at least one construct expressing at least a portion of a flavanone 3-hydroxylase, a C1 myb transcription factor, and an R-type myc transcription factor that regulate expression of genes in the phenylpropanoid pathway.
Claims35
A method of increasing isoflavonoid production in an isoflavonoid-producing plant, the method comprising:
- a) transforming a plant with (1) a first recombinant DNA construct comprising a polynucleotide selected from the group consisting of:
- (i) a polynucleotide encoding all or part of a flavanone 3-hydroxylase;
- (ii) a polynucleotide from 5′ and/or 3′ ends of an isolated nucleic acid fragment which encodes flavanone 3-hydroxylase;
- or
- (iii) a polynucleotide comprising (i) and (ii);
- and (2) at least one second recombinant DNA construct comprising a polynucleotide encoding a C1 myb transcription factor and a R myc-type transcription factor;
- and
- b) growing the transformed plant of (a);
- and
- c) evaluating the plant or plant part obtained from the transformed plant for an increased quantity of isoflavonoid in the transformed plant or plant part as compared plant or plant parts obtained from an untransformed plant.
7 more elements
An isoflavonoid-producing plant comprising in its genome
- (1) a first recombinant DNA construct comprising a polynucleotide selected from the group consisting of:
- (i) a polynucleotide encoding all or part of a flavanone 3-hydroxylase;
- (ii) a polynucleotide from 5′ and/or 3′ ends of an isolated nucleic acid fragment which encodes flavanone 3-hydroxylase;
- or
- (iii) a polynucleotide comprising (i) and (ii);
- and
- (2) at least one second recombinant DNA construct comprising a polynucleotide encoding a C1 myb transcription factor and a R myc-type transcription factor;
- wherein the plant or plant parts obtained from the transformed plant have an increased quantity of isoflavonoid as compared to the plant or plant parts obtained from an untransformed plant.
5 more elements
Dependencies are inferred from the claim text.