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AssigneeAgriculture and Agri-Food Canada
InventorsMargaret Y. Gruber, Heather Ray
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The present invention provides two novel regulatory genes and encoded proteins which can be used to alter the biosynthesis and accumulation of condensed tannin levels in plants and plant tissues. The present invention further encompasses transgenic constructs containing the novel regulatory genes herein referred to as Ulimyc, Corniculmyc and Japmyc, for use in the transformation of plants and plant tissues and transgenic plants containing such constructs. The identification and characterization of these novel genes provide a mechanism for altering tannin production in plants and allows one to alter such levels to produce a variety of benefits in the field of agriculture, land reclamation animal farming and food technology in general.
An isolated nucleic acid comprising:
An isolated nucleic acid comprising an antisense nucleotide sequence selected from the group consisting of:
A PCR primer comprising a nucleic acid sequence of SEQ ID NO:
A substantially purified protein encoded by any one of the amino acid sequences of Table 4 (SEQ ID NO:
A substantially purified protein comprising an amino acid sequence having at least 80% amino acid identity to the amino acid sequence of Table 4 (SEQ ID NO:
A method for producing the proteins encoded by the Ulimyc, Japmyc or Corniculymc genes, comprising culturing host cells to permit expression of a Ulimyc, Japmyc or Corniculmyc protein-encoding nucleic acid sequence and production of the protein.
A method for suppressing or reducing condensed tannin synthesis in a plant or plant tissue comprising transforming a plant or plant tissue with an isolated nucleic acid comprising the nucleic acid sequence of Table 3 (SEQ ID NO:3).
A method for altering the condensed tannin level in a plant or plant cell comprising transforming or treating the selected plant or plant cell with a substance selected from the group consisting of:
An isolated nucleic acid molecule comprising a nucleic acid sequence encoding a protein or peptide capable of altering the production of condensed tannins or anthocyanins in plant cells and tissues expressing such, wherein the nucleic acid sequence is selected from the group consisting of:
An isolated nucleic acid molecule comprising a nucleic acid sequence, wherein said isolated nucleic acid molecule, when introduced into a plant cell, has a negative effect on the expression of condensed tannins and/or anthocyanins, wherein the nucleic acid sequence is selected from the group consisting of:
A transgenic plant cell comprising a DNA molecule introduced into said plant cell, wherein said DNA molecule is selected from the group consisting of:
A transgenic plant comprising a recombinant nucleic acid sequence of SEQ ID NO:
Dependencies are inferred from the claim text.