Endoglucanase gene promoter upregulated by the root-knot nematode
AssigneeNorth Carolina State University
InventorsEric L. Davis, Melissa Goellner
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Abstract
The present invention provides a DNA construct comprising a root knot nematode responsive promoter, preferably the Arabidopsis cell promoter or promoters that hybridize thereto, operatively associated with a heterologous DNA segment that encodes a product disruptive of nematode attack. Plants and plant cells using the same and methods of use thereof are also disclosed.
Claims23
A DNA construct which comprises, in the 5′ to 3′ direction:
- (a) a root knot nematode responsive promoter selected from the group consisting of:
- (i) DNA encoding the Arabidopsis cell promoter according to SEQ ID NO:1, and (ii) DNA sequences which hybridize to DNA having a sequence of (i) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, 0.1% SDS at 60°, and which encodes a root knot nematode responsive promoter;
- and
- (b) a heterologous DNA positioned downstream from said root knot nematode promoter and operatively associated therewith, said heterologous DNA encoding a product disruptive of nematode attack.
A DNA construct according to claim 4, wherein said heterologous DNA segment is a gene coding for a Bacillus thuringiensis crystal protein toxic to insects.
A transformed plant according to claim 1515, wherein said plant is a dicot.
A method of controlling root knot nematodes, comprising the steps of:
- (a) providing a root knot nematode-responsive promoter selected from the group consisting of:
- (i) DNA encoding the Arabidopsis cell promoter of SEQ ID NO:1, and (ii) DNA sequences which hybridize to DNA having a sequence of (i) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, 0.1% SDS at 60°, and which encodes a root knot nematode-responsive promoter;
- (b) preparing a construct by combining said promoter with a further heterologous region which codes for a product disruptive of nematode attack, and
- (c) transforming plants with the construct to obtain plants which are root knot nematode resistant.
Dependencies are inferred from the claim text.