US 2004/0006790 A1Published application

Method for producing plants which are resistant to closteroviruses

AssigneeDe Ruiter Seeds C.V. LP

InventorsWouter Pieter Johannes De Ruiter, Bernardus Josef Van Der Knaap, Abraham Alexander Klapwijk, Rene Johannes Maria Hofstede

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Abstract

A method for producing cucumber plants which are resistant to cucumber closteroviruses. The method comprises the steps of providing a Cucumis sativus plant which contains alleles that confer resistance to the closteroviruses defined by two QTLs, crossing said C. sativus plant with C. sativus culture breeding material, collecting the seeds resulting from said cross, regenerating the seeds into plants, evaluating the plants for resistance to the closteroviruses, and identifying and selecting resistant plants. Further, a resistant cucumber plant produced by the method as well as fruit or seed produced by said plant.

Claims12

Dependent claims

A method for producing cucumber plants which are resistant to closteroviruses occurring in cucumber, the method comprising the steps of:

a. providing a Cucumis sativus plant which contains alleles that confer resistance to cucumber closteroviruses, which alleles are characterized by two Quantitative Trait Loci QTLI and QTL2 on different chromosomes, wherein QTL1 is defined by the following flanking markers:
i) a marker of about 244 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
1, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
2,
ii) a marker of about 188 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
1, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
2, and
iii) a marker of about 251 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
3, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
4,
or any part of the DNA sequence as in PI250147 between the flanking markers conferring closterovirus resistance,
and QTL2 is defined by the following markers:
i) a marker of about 260 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
5, a cucumber genomic fragment and a MseI primer having the nucleotide sequence ID NO:
2, and
ii) a marker of about 107 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
5, a cucumber genomic fragment and a MSeI primer having the nucleotide sequence SEQ ID NO:
6,
or any part of the DNA sequence as in PI250147 between the flanking markers conferring closterovirus resistance,
b. crossing the Cucumis sativus plant provided in step a with Cucumis sativus culture breeding material,
c. collecting the seeds resulting from the cross in step b,
d. regenerating the seeds into plants,
e. evaluating the plants of step d for resistance to cucumber closteroviruses, and
f. identifying and selecting plants which are resistant to the cucumber closteroviruses, using the markers as defined in step a.

21 more elements

A method for producing seeds that result in cucumber plants resistant to closteroviruses occurring in cucumber, the method comprising the steps of:

a. providing a Cucumis sativus plant which contains alleles that confer resistance to cucumber closteroviruses, which alleles are characterized by two Quantitative Trait Loci QTL1 and QTL2 on different chromosomes, wherein QTL1 is defined by the following flanking markers:
i) a marker of about 244 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
1, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
2, I
ii) a marker of about 188 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence StQ ID NO:
1, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
2, and
iii) a marker of about 251 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
3, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
4,
or any part of the DNA sequence as in PI250147 between the flanking markers conferring closterevirus resistance,
and QTL2 is defined by the following markers:
i) a marker of about 260 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
5, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
2, and
ii) a marker of about 107 bp consisting from 5′ to 3′ of an EcoRI primer having the nucleotide sequence SEQ ID NO:
5, a cucumber genomic fragment and a MseI primer having the nucleotide sequence SEQ ID NO:
6,
or any part of the DNA sequence as in PI250147 between the flanking markers conferring closterovirus resistance,
b. crossing the Cucumis sativus plant provided in step a with Cucumis sativus culture breeding material,
c. collecting seeds resulting from the cross in step b,
d. selecting and identifying seeds that result in cucumber plants which are resistant to cucumber closteroviruses, using the markers as defined in step a.

19 more elements

Dependencies are inferred from the claim text.

Facts

Application: 10/386,397Filed 11 Mar 2003
Priority: EP 00 203 191.214 Sep 2000
Published: 8 Jan 2004
Term ends: Not estimatedThe source records don’t support an estimate.

Classification4 codes

A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 5/08
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: fruits
A01H 6/346
Cucurbitaceae, e.g. bitter melon, cucumber or watermelon: cucumis sativus[cucumber]
A01H
New plants or non-transgenic processes for obtaining them