Non-human mammal mutant for expression of the alpha6 subunit of the nicotinic acetylcholine receptor, and use for screening substances that can interact with said receptor
AssigneeInstitut Pasteur
InventorsNicolas Champtiaux, Jean-Pierre Changeux, Alain Bessis
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Abstract
The invention concerns a non-human mammal carrying a mutation in the gene coding for the alpha6 subunit of the nicotinic acetylcholine receptor (nAChR), said mutation preventing expression of said nAChR alpha6 subunit in a functional form in the mammal. The invention also concerns synaptosome preparations obtained from said animals and cell cultures obtained by mutation of the alpha6 subunit as defined above.
Claims33
A non-human mammal carrying a mutation in the gene coding for the alpha6 subunit of the nicotinic acetylcholine receptor (nAChR), said mutation preventing expression in the mammal of said nAChR alpha6 subunit in a functional form.
A plasmid containing all or part of the gene coding for the mouse nicotinic acetylcholine receptor alpha6 subunit, selected from the following plasmids deposited at the CNCM:
- a6HN:
- CNCM N° I-2500 deposited on Jun. 22, 2000;
- a6EH:
- CNCM N° I-2501 deposited on Jun. 22, 2000;
- a6EE:
- CNCM N° I-2502 deposited on Jun. 22, 2000;
- a6PP:
- CNCM N° I-2503 deposited on Jun. 22, 2000;
- a6KO:
- CNCM N° I-2550 deposited on Aug. 29, 2000;
- or the bacterial artificial chromosome (BAC) designated a6BAC deposited at the CNCM with accession number I-2504 on Jun. 22, 2000.
A nucleotide sequence, characterized in that it is a sequence of the gene coding for the nAChR alpha6 subunit contained in a plasmid selected from a6HN (CNCM, I-2500), a6EH (CNCM, I-2501), a6EE (CNCM, I-2502), a6PP (CNCM, I-2503), a6KO (CNCM, I-2550) or in the bacterial artificial chromosome a6BAC (CNCM, I-2504).
A nucleotide sequence selected from the concatenations represented in sequences SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6 or any part of said concatenations corresponding to a sequenced fragment of the gene for the nAChR alpha6 subunit.
Substances obtained from a screening method according to any one of clams 20 to 25 or 32.
Dependencies are inferred from the claim text.