Flagellin gene, flaC of Campylobacter
AssigneeUniversity of Toronto
InventorsVoon Loong Chan, Helena Louie
Life of this patent
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Abstract
Purified and isolated nucleic acid molecules are provided which encode a FlaC flagellin protein of a strain of Campylobacter, particularly C. jejuni, or a fragment or an analog of the FlaC flagellin protein. The nucleic acid molecules may be used to produce proteins free of contaminants derived from bacteria normally containing the FlaA or FlaB proteins for purposes of diagnostics and medical treatment. Furthermore, the nucleic acid molecules, proteins encoded thereby and antibodies raised against the proteins, may be used in the diagnosis of infection.
Claims28
A purified and isolated nucleic acid molecule encoding a flagellin protein C (FlaC) of a flagellum of a strain of Campylobacter or a fragment or an analog of the FlaC protein.
A purified and isolated nucleic acid molecule having a nucleotide sequence selected from the group consisting of:
- (a) the entire nucleotide sequence set out in FIG. 1 (SEQ ID No:
- 1), or the complementary sequence of said sequence;
- (b) the coding nucleotide sequence set out in FIG. 1 (SEQ ID No:
- 2), or the complementary sequence of said sequence;
- (c) a nucleotide sequence encoding the amino acid sequence set forth in FIG. 1 (SEQ ID No:
- 3);
- and
- (d) a nucleotide sequence which hybridizes under stringent conditions to any one of the sequences defined in (a), (b) or (c).
The nucleic acid molecule of claim 5, wherein the nucleotide sequence defined in (b) has at least about 90% sequence identity with any one of the sequences defined in (a).
An isolated and purified FlaC protein of Campylobacter strain substantially free from other proteins of the Campylobacter strain.
A synthetic peptide having no less than six amino acids and no more than 150 amino acids and containing an amino acid sequence corresponding to a portion only of a FlaC protein of a strain of Campylobacter or of an analog of the FlaC protein.
An immunogenic composition, comprising at least one active component selected from the group consisting of:
- (A) a purified and isolated nucleic acid molecule encoding a flagellin protein C (FlaC) of a flagellum of a strain of Campylobacter or a fragment or an analog of the FlaC protein;
- (B) a purified and isolated nucleic acid molecule having a sequence selected from the group consisting of:
- (a) the entire nucleotide sequence set out in FIG. 1 (SEQ ID No:
- 1), or the complementary sequence of said sequence;
- (b) the coding nucleotide sequence set out in FIG. 1 (SEQ ID No:
- 2), or the complementary sequence of said sequence;
- (c) a nucleotide sequence encoding the amino acid sequence set forth in FIG. 1 (SEQ ID No:
- 3);
- and
- (d) a nucleotide sequence which hybridizes under stringent conditions to any one of the sequences defined in (a), (b) or (c).
- (C) a recombinant FlaC protein or fragment or analog thereof producible in a transformed host containing an expression vector comprising a nucleic acid molecule as defined in (A) or (B) and expression means operatively coupled to the nucleic acid molecule for expression by the host of the recombinant FlaC protein or fragment or analog thereof;
- (D) an isolated and purified FlaC protein of a Campylobacter strain substantially free from other proteins of the Campylobacter strain;
- (E) a live vector, comprising a vector containing a purified and isolated nucleic acid molecule encoding a FlaC protein of a flagellum of a strain of Campylobacter or a fragment or an analog of the FlaC protein;
- (F) a live vector, comprising a vector containing a purified and isolated nucleic acid molecule having a sequence selected from the group consisting of:
- (a) the entire nucleotide sequence set out in FIG. 1 (SEQ ID No:
- 1), or the complementary sequence of said sequence;
- (b) the coding nucleotide sequence set out in FIG. 1 (SEQ ID No:
- 2), or the complementary sequence of said sequence;
- (c) a nucleotide sequence encoding the amino acid sequence set forth in FIG. 1 (SEQ ID No:
- 3);
- and
- (d) a nucleotide sequence which hybridizes under stringent conditions to any one of the sequences defined in (a), (b) or (c).
- (G) a synthetic peptide having no less than six amino acids and no more than 150 amino acids and containing an amino acid sequence corresponding to a portion only of a FlaC protein of a strain of Campylobacter or of an analog of the FlaC protein;
- and a pharmaceutically acceptable carrier therefor;
- said at least one active component producing an immune response when administered to a host.
Dependencies are inferred from the claim text.