US 2004/0005710 A1 · Method for producing recombinant viruses using site-specific recombination

Abstract

Disclosed are the methods for producing recombinant viruses using in vitro site-specific recombination. In the present invention, linear viral genomic DNAs are digested to two segments, and are subjected to in vitro site-specific recombination with desired genomic materials flanked with site-specific recombinase sites. According to the present invention, since the site-specific recombination mixture can be applied to host cells without further procedures of selecting the recombinant viral genomic DNAs, it is possible to obtain numerous recombinant viruses rapidly at the same time. Thus, the present invention can be used as a high throughput system to generate hundreds or thousands of recombinant viruses.

Claims14

Dependent claims

A method for preparing linear recombinant viral vectors, wherein desired DNAs are inserted into viral genomic DNAs by in vitro site-specific recombination, which comprises:

i) providing viral genomic DNA segments containing site-specific recombination sequences and base sequences essential to viral replication and packaging,
ii) providing carrier vectors comprising at least one desired DNAs and site-specific recombination sequences;
and
iii) reacting the DNA segments of i) and the carrier vectors of ii) in vitro.

1 more element

Dependencies are inferred from the claim text.

Method for producing recombinant viruses using site-specific recombination

Life of this patent

Method for producing recombinant viruses using site-specific recombination

Life of this patent

Abstract

Claims14