Insulin producing cell-line
OwnerPeter R Flatt, Neville H McClenaghaninventors
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Abstract
The invention provides insulin secreting human pancreatic cell lines and a process for their production from the electrofusion of normal human islet cells with immortal human cell lines to generate hybrid fusion cells which are able to produce insulin. The invention also provides uses for cell-lines.
Claims21
A human pancreatic cell line produced by electrofusion of normal human islet cells with cells from at least one immortal human cell line wherein the human pancreatic cell-line is capable of secreting insulin.
A human pancreatic cell line capable of secreting insulin chosen from the group of cell lines consisting of the cell-line deposited under Accession No 00112811 at the European Collection of Cell Cultures (ECACC), CAMR, Salisbury, Wiltshire on Nov. 28, 2000 and the cell-lines deposited under Accession Nos PTA 3523, PTA 3524 and PTA 3525 at the American Type Culture Collection, 10801 University Boulevard, Manassas, Va. 20100-2209, USA on Jul. 17, 2001.
A process for the production of human pancreatic cell lines capable of secreting insulin, the process including the steps of electrofusing a mixture of normal human islet cells with cells from at least one immortal human cell line and incubating the mixture to generate hybrid cells.
Dependencies are inferred from the claim text.