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US 2004/0005697 A1Published application
Stage and platform for building a biochip and biochip
AssigneeAutogenomics, Inc.
InventorsVijay K. Mahant, Fareed Kureshy
Life of this patent
Estimating the term…
Abstract
A biochip, a platform which composes the biochip, and a stage which composes the platform. The biochip is for detecting analytes in a test sample. The platform comprises a stage. The stage of the invention includes a carrier.
Claims20
A biochip comprising:
- a carrier with a hydrophilic surface;
- at least one light-blocking matrix layer comprising a light-blocking agent, wherein the at least one light-blocking matrix layer is coupled to the hydrophilic surface and is effective to render the carrier optically inactive;
- a coupling matrix layer coupled to the light-blocking matrix layer, and a cross-linking agent physically suspended in the coupling matrix layer, wherein the cross-linking agent is optionally covalently coupled to the coupling matrix layer;
- a plurality of sensing elements, each of the plurality of sensing elements having a capture portion that binds to the cross-linking agent, and wherein the sensing elements are bound to the cross-linking agent in the coupling matrix layer in predetermined positions;
- and
- a modification matrix layer coupled to the coupling matrix layer, wherein the modification matrix layer provides at least one of a chromatographic function, a light absorbing function, a penetration delay function, and an assay function.
3 more elements
A biochip comprising:
- a carrier with a first and second surface, wherein at least one of the first and second surfaces is hydrophilic or wherein the first surface has a hydrophilic coating;
- a base matrix layer coupled to the hydrophilic coating or hydrophilic surface;
- a light-blocking matrix layer coupled to the base matrix layer and comprising a light-blocking agent, wherein the light-blocking matrix layer is coupled to the base matrix layer and is effective to render the carrier optically inactive;
- a coupling matrix layer coupled to the light-blocking matrix layer, and a cross-linking agent physically suspended in the coupling matrix layer, wherein the cross-linking agent is optionally covalently coupled to the coupling matrix layer;
- a plurality of sensing elements, each of the plurality of sensing elements having a capture portion that binds to the cross-linking agent, and wherein the sensing elements are bound to the cross-linking agent in the coupling matrix layer in predetermined positions;
- wherein at least one of the base layer, the light-blocking matrix layer, and the coupling matrix layer comprises an additive selected from the group consisting of a buffer, a detergent, a humectant, and a light-blocking agent;
- and
- wherein the plurality of sensing elements are coupled to the coupling matrix layer via droplet deposition of liquid droplets having a diameter of between 20 and 1000 micrometer to form a corresponding plurality of sensing element spots.
5 more elements
A biochip comprising:
- a carrier with a first and second surface, and a hydrophilic coating coupled to the first surface of the carrier;
- at least one light-blocking matrix layer comprising a light-blocking agent, wherein the at least one light-blocking matrix layer is coupled to the hydrophilic coating and is effective to render the carrier optically inactive;
- a coupling matrix layer coupled to the light-blocking matrix layer, and a cross-linking agent physically suspended in and on the coupling matrix layer, wherein the cross-linking agent is optionally covalently coupled to the coupling matrix layer;
- a plurality of sensing elements, each of the plurality of sensing elements having a capture portion that binds to the cross-linking agent, and wherein the sensing elements are bound to the cross-linking agent in the coupling matrix layer in predetermined positions;
- and
- wherein the plurality of sensing elements are coupled to the coupling matrix layer via droplet deposition of liquid droplets having a diameter of between 20 and 1000 micrometer to form a corresponding plurality of sensing element spots.
3 more elements
Dependencies are inferred from the claim text.