Modified BACE
AssigneeElan Pharmaceuticals, Inc.
InventorsKuo-Chen Chou, W. Jeffrey Howe
Life of this patent
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Abstract
The present invention relates to recombinant human BACE polypeptides. More particularly, the invention relates to recombinant human BACE polypeptides that have a modified amino acid sequence at position 33 of the BACE sequence, as well as to polynucleotides, expression vectors, host cells, and methods for producing the modified recombinant human BACE polypeptides.
Claims21
An isolated polypeptide comprising human BACE having the modification Pro33Lys.
A composition comprising an active human BACE enzyme comprising the pro-enzyme sequence of BACE having the modification Pro33Lys.
An isolated polypeptide comprising SEQ ID NO:
- 2.
An isolated polypeptide consisting of SEQ ID NO:
- 2.
An isolated polynucleotide comprising a sequence that encodes the amino acid sequence set forth in SEQ ID NO:
- 2.
An isolated polynucleotide comprising nucleotides 70-1365 of SEQ ID NO:
- 8.
An isolated polynucleotide consisting of nucleotides 70-1365 of SEQ ID NO:
- 8.
An expression vector comprising a polynucleotide sequence encoding for a Pro33Lys-BACE polypeptide, wherein said expression vector can produce the Pro33Lys-BACE polypeptide when said expression vector is present in a compatible host cell, and when the host cell is cultured under conditions that allow for production.
Dependencies are inferred from the claim text.