Heterologous production of polyketides
OwnerDaniel V. Santi, Chaitan Khoslainventors
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Abstract
Recombinant host cells that comprise recombinant DNA expression vectors that drive expression of a product and a precursor for biosynthesis of that product can be used to produce useful products such as polyketides in host cells that do not naturally produce the product or produce the product or precursor at low levels due to the absence of the precursor or the presence of the precursor in rate limiting amounts.
Claims20
A recombinant host cell comprising one or more expression vectors that drive expression of enzymes capable of making a product and a precursor required for biosynthesis of the product in said host cell, wherein said host cell, in the absence of said expression vectors, is unable to make said product due to lacking all or a part of a biosynthetic pathway required to produce the precursor.
A recombinant host cell comprising one or more expression vectors that drive expression of enzymes capable of making a product and a precursor required for biosynthesis of the product in said host cell, wherein said host cell, in the absence of said expression vectors for said enzymes capable of making said precursor, makes said product in substantially lesser amounts due to said precursor being present in said host in limiting amounts.
An E. coli host cell that expresses heterologous methylmalonyl CoA mutase and epimerase genes.
Dependencies are inferred from the claim text.