Recombinant expression of insulin C-peptide
OwnerStefan Stahl, Mathias Uhlen +2inventors
InventorsStefan Stahl, Mathias Uhlen, Per-Ake Nygren, Per Jonasson
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Abstract
The present invention provides a method of producing an insulin C-peptide, which comprises expressing in a host cell a multimeric polypeptide comprising multiple copies of a said insulin C-peptide, and cleaving said expressed polypeptide to release single copies of the insulin C-peptide. Also provided are nucleic acid molecules, expression vectors and host cells, for use in such a method and the multimeric insulin C-peptide poolypeptide expressed and cleaved in such a method.
Claims50
A method of producing an insulin C-peptide, which comprises expressing in a host cell a multimeric polypeptide comprising multiple copies of a said insulin C-peptide, and cleaving said expressed polypeptide to release single copies of the insulin C-peptide.
A nucleic acid molecule comprising multiple copies of a nucleotide sequence encoding an insulin C-peptide, wherein said nucleic acid molecule encodes a multimeric polypeptide capable of being cleaved to yield single copies of said insulin C-peptide.
A method for the production of a nucleic acid molecule which encodes a multimeric polypeptide comprising multiple copies of an insulin C-peptide, wherein the expressed multimeric polypeptide is capable of being subsequently cleaved to yield single copies of the insulin C-peptide, said process comprising generating a nucleic acid molecule comprising multiple copies of a nucleotide sequence encoding an insulin C-peptide, linked in matching reading frame.
A multimeric polypeptide comprising multiple copies of an insulin C-peptide, wherein said multimeric polypeptide can be cleaved to release single copies of said insulin C-peptide.
A method of producing a multimeric polypeptide which contains multiple copies of an insulin C-peptide and can be cleaved to release single copies of said insulin C-peptide, said method comprising culturing a host cell containing a nucleic acid molecule encoding said multimeric polypeptide under conditions whereby said multimeric polypeptide is expressed, and recovering the expressed multimeric polypeptide.
Dependencies are inferred from the claim text.