Method of making CS6 antigen vaccine for treating, preventing, or inhibiting enterotoxigenic Escherichia coli infections
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsFrederick J. Cassels, James F. Wood
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Abstract
Disclosed herein are methods for making large amounts of highly pure colonization factors. The methods of the present invention differ from prior art methods in that host cells which express the colonization factor of interest are cultured in media comprising more than about 50 μg/l of an antibiotic, the media is centrifuged and then filtered with a 0.2 μm filter tangential flow cartridge and a 300,000 MW cut-off filter, and a divalent cation is added. As disclosed herein the colonization factors made by the method of the present invention may be used in pharmaceutical compositions and methods for treating or preventing enterotoxigenic Escherichia coli infections.
Claims47
A method of making a preparation containing an amount of at least one colonization factor which comprises
- culturing a host cell comprising a nucleotide sequence that encodes the colonization factor and a gene for resistance to an antibiotic in a growth medium having more than about 50 μg/ml of the antibiotic;
- centrifuging the growth medium;
- and
- filtering the growth medium.
An oral pharmaceutical formulation comprising microencapsulated CS6 in a therapeutically effective amount.
Dependencies are inferred from the claim text.