Potential growth factors from the human tumour cell line ht 1080
OwnerStephen L. Minger, Gregor Adams +2inventors
InventorsStephen L. Minger, Gregor Adams, Paul Francis, Myra Mcclure
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Abstract
The invention relates to a mitogen obtainable from a human tumour cell line, such as from HT1080 cells.
Claims25
An isolated or purified mitogen obtainable from a human tumour cell line.
Conditioned medium comprising a mitogen, said medium being conditioned via the growth of a human tumour cell line therein.
Conditioned medium comprising a mitogen, said medium being conditioned via the growth of a human tumour cell line.
An isolated or purified mitogen obtained from a human tumour cell line.
A mitogen obtainable from the medium in which a human tumour cell line has been cultured.
A stem cell mitogen obtainable from human tumour cell line.
A stem cell mitogen obtainable from the medium in which a human tumour cell line has been cultured.
A neural stem cell mitogen obtainable from human tumour cell line.
A neural stem cell mitogen obtainable from the medium in which a human tumour cell line has been cultured.
A mitogen according to any one of the preceding claims wherein the mitogen is NSC-MF-1 and/or NSC-MF-2.
A mitogen according to any one of the preceding claims wherein the mitogen is obtainable from a method comprising the use of sequential Sepharose 4B and anion exchange chromatographic techniques to at least partially purify the mitogen from other contaminating proteins.
A method for culturing eukaryotic cells in vitro in a medium comprising a mitogen according to any previous claim.
A method for culturing eukaryotic cells in vitro in a medium comprising an exogenously added mitogen according to any previous claim.
A method for culturing eukaryotic cells in vitro in a medium comprising exogenously added conditioned medium according to any previous claim.
A method for promoting cell survival comprising culturing said cell in medium comprising an exogenously added mitogen according to any previous claim.
A method for promoting cell survival comprising culturing said cell in medium comprising exogenously added conditioned medium according to any previous claim.
A mitogen according to any previous claim wherein said mitogen is a protein or fragment thereof.
A mitogenic composition comprising a mitogen according to any previous claim, and/or comprising conditioned medium according to any previous claim.
A method for the preparation of a mitogenic composition comprising
- (i) providing a suitable diluent
- (ii) adding a mitogen and/or conditioned medium according to any previous claim thereto.
A method for modulating the mitogenic effect of a first mitogen comprising adding a suitable amount of a second mitogen according to any previous claim.
A method for augmenting the mitogenic effect of a factor selected from GMCSF, LIF, EGF, FGF, IGF-1, FGF-8, thrombopoietin or neurotrophin-3, comprising adding an augmenting amount of a mitogen according to any previous claim.
A method for propagating a cell, said method comprising incubating said cell in a medium comprising a mitogen according to any previous claim.
A method for potentiating neural cell growth as shown in the accompanying drawings comprising the use of a mitogen according to the invention as described herein.
Use of a mitogen according to any previous claim to selectively potentiate the proliferation of progenitor cells that give rise to an increase in neurons.
A method of increasing the proportion of neurons developing from a population of neural stem cells, said method comprising contacting said population of neural stem cells with a mitogen according to any previous claim.
Dependencies are inferred from the claim text.