Human PRSS11-like S2 serine protease and uses thereof
OwnerAndrew Lawrence Darrow, Jian-Shen Ql +2inventors
InventorsAndrew Lawrence Darrow, Jian-Shen Ql, Cailin Chen, Patricia Andrade-Gordon
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Abstract
A novel human cDNA, termed PRSS11-L, was isolated which encodes a polypeptide that belongs to the S2/HtrA serine protease family. The PRSS11-L mRNA is widely expressed in several tissues throughout the body by multi-tissue Northern blotting. The full-length PRSS11-L cDNA, was cloned, expressed and purified. Proteolytic activity was demonstrated using the protein substrate casein. The isolated nucleic acid or polypeptide molecule of the invention can be used in detection assays, gene therapy, and screening assays.
Claims24
An isolated nucleic acid molecule capable of encoding an S2 serine protease, wherein the isolated nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule comprising a nucleotide sequence encoding a polypeptide which has at least a 90% sequence identity to the amino acid 1 to 9 of SEQ ID NO:
- 2
- (b) a nucleic acid molecule comprising at least 12 sequential nucleotides of SEQ ID NO:
- 1 from nucleotide 1 to 1038;
- (c) a nucleic acid molecule having at least a 70% sequence identity to SEQ ID NO:
- 1 from nucleotide 1 to 1038;
- and
- (d) a nucleic acid molecule which is complementary to the sequence of (a), (b), or (c).
A substantially purified polypeptide having S2 serine protease activity, comprising an amino acid sequence having at least a 90% identity to amino acid 1 to 9 of SEQ ID NO:
- 2.
An antibody that selectively binds polypeptides with amino acid sequences substantially similar to the amino acids 1 to 9 of SEQ ID NO:
- 2.
A method of increasing the expression of PRSS11-L in a cell of a subject in need thereof, comprising the step of administering to the subject a nucleic acid molecule capable of expressing a functional PRSS11-L protein in the cell.
A method of identifying a compound that increases or decreases the expression of a PRSS11-L protein, comprising the steps of:
- (a) contacting a test compound with a regulatory sequence for a PRSS11-L gene or with a cellular component that binds to the regulatory sequence for a PRSS11-L gene;
- and
- (b) determining whether the test compound increases or decreases the expression of a gene controlled by said regulatory sequence.
A method of identifying a compound that increases or decreases a biological activity of a PRSS11-L protein, comprising the steps of:
- (a) contacting a test compound with a PRSS11-L protein;
- and
- (b) determining whether the test compound increases or decreases the biological activity of PRSS11-L.
A method of identifying a compound that increases or decreases the protease activity of PRSS11-L, comprising the steps of:
- (a) contacting a test compound with a PRSS11-L protein and with a substrate that is cleavable by the PRSS11-L protease;
- and
- (b) determining whether the test compound increases or decreases the cleavage of said substrate by the PRSS11-L protease.
A method of identifying a compound that binds to a PRSS11-L protein, comprising the steps of:
- (a) incubating a test compound with a PRSS11-L protein and a labeled ligand for the PRSS11-L protein;
- (b) separating the PRSS11-L protein from unbound labeled ligand;
- and
- (c) identifying a compound that inhibits ligand binding to the subunit by a reduction in the amount of labeled ligand binding to the PRSS11-L.
Dependencies are inferred from the claim text.