Method of producing polyvalent antigens
OwnerSzu-Yi Chouinventor
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Abstract
Embodiments of the invention generally provide methods and compositions for producing polyvalent antigens. In one aspect, the invention provides a method for producing a cross-linked antigen. In another aspect, the invention provides a method of using cross-linked products as antigens to immunize animals and induce strong immune responses.
Claims80
A method for producing a polyvalent antigen using a biological agent, comprising:
- preparing an antigen in a cross-linking solution;
- combining the antigen cross-linking solution with a solution of the biological agent into a mixture;
- and
- incubating the mixture at a temperature for a period of time sufficient to effect cross-linking of the antigen into a plurarilty of cross-linked products;
- and
- providing the cross-linked products to be used as the polyvalent antigen.
A method for producing a polyvalent antigen using a transglutaminase, comprising:
- preparing an antigen in a cross-linking solution;
- combining the antigen cross-linking solution with a solution of the transglutaminase into a mixture;
- incubating the mixture for a period of time at a temperature sufficient to effect cross-linking of the antigen into a plurality of cross-linked products;
- and
- providing the cross-linked products to be used as the polyvalent antigen.
A method for preparing an antigen having immunogenic activity, comprising:
- preparing a compound in a cross-linking solution;
- combining the compound cross-linking solution with a biological agent into a mixture;
- incubating the mixture at a temperature for a period of time sufficient to effect cross-linking of the compound into a plurality of cross-linked products;
- and
- providing the cross-linked products to be used as the antigen.
A method for preparing an antigen having immunogenic activity, comprising:
- preparing two or more compounds;
- combining the two or more compounds in a cross-linking solution with a biological agent into a mixture;
- incubating the mixture at a temperature for a period of time sufficient to effect cross-linking of the two or more compounds into a plurality of cross-linked products;
- and
- providing the cross-linked products to be used as the antigen.
A method for preparing a vaccine having immunogenic activity, comprising:
- preparing a compound in a cross-linking solution;
- combining the compound cross-linking solution with a biological agent into a mixture;
- incubating the mixture for a period of time at a temperature sufficient to effect cross-linking of the compound into a plurality of cross-linked products;
- and
- providing the plurality of cross-linked products to be used as the vaccine.
A method for producing an antibody specifically for a compound, comprising:
- preparing the compound in a cross-linking solution;
- combining the compound cross-linking solution with a biological agent into a mixture;
- incubating the mixture at a temperature for a period of time sufficient to effect cross-linking of the compound into a plurality of cross-linked products;
- immunizing an animal with the plurality of cross-linked products under conditions to elicit an antibody response;
- and
- isolating antibodies produced by the animal.
An antigen composition, comprising:
- a plurality of cross-linked products prepared by combining a compound with a solution of a biological agent in a cross-linking solution into a mixture at a temperature for a period of sufficient time.
A cross-linking solution composition for cross-linking a polypeptide by an biological agent, comprising:
- at least one reducing agent;
- up to about 50% of glycerol;
- deionized water;
- and
- a pH buffering agent for adjusting the pH of the composition.
Dependencies are inferred from the claim text.