Methods and devices for the detection of pathogenic microorganisms and their antimicrobial susceptibility

A method of providing a service for determining the presence or absence of pathogens in a biological sample and for determining the antimicrobial susceptibility of pathogens present in the sample comprising:

• collecting a biological sample;
• using an assay device to preliminarily determine the presence or absence of pathogens in the sample and to concurrently preliminarily determine the antimicrobial susceptibility of pathogens present in the sample;
• obtaining a result of confirmatory testing of the presence or absence of pathogens in the sample and obtaining a result of confirmatory testing of the antimicrobial susceptibility of pathogens present;
• thereby providing the service of determining the presence or absence of pathogens in a biological sample and of determining the antimicrobial susceptibility of pathogens present.

A kit comprising:

• an assay device for detecting the presence or absence of primary gram negative uropathogens in a biological sample and for concurrently determining the antimicrobial susceptibility of uropathogens present;
• and one or more of the following items,
• a moisture impervious enclosure enclosing the device;
• instructions for applying a sample suspected of containing uropathogens to the device;
• and
• at least one pipette;
• at least one container of a substance that increases a fluorescent signal when added to a well of the device;
• and
• optionally comprising an outer enclosure enclosing the assay device in a moisture impervious enclosure.