Chromogenic plating medium for the rapid presumptive identification of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiesis
AssigneeA. R. F. Products, Inc.
InventorLawrence Restaino
Life of this patent
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Abstract
A plating medium for the presumptive identification of Bacillus cereus, Bacillus thuringiensis and/or Bacillus anthrasis that includes a nutrient base to facilitate growth of Bacillus cereus, Bacillus thuringiensis and Bacillus anthrasis, a chromogenic substrate that changes color responsive to the presence of PC-PLC enzymes, and an ingredient that promotes the expression of the PC-PLC enzyme. In a preferred embodiment, the medium includes a second chromogenic substrate that changes color responsive to the presence of PI-PLC enzymes and an ingredient that promotes the expression of the PI-PLC enzyme.
Claims18
A plating medium for the presumptive identification of Bacillus cereus, Bacillus thuringiensis and/or Bacillus anthrasis comprising a nutrient base to facilitate growth of Bacillus cereus, Bacillus thuringiensis and Bacillus anthrasis, at least one member of a group consisting of a first chromogenic substrate that changes color responsive to the presence of PC-PLC enzymes, and a second chromogenic substrate that changes color responsive to the presence of PI-PLC enzymes, and at least one ingredient that promotes the expression of the PC-PLC and PI-PLC enzymes.
A plating medium for the presumptive identification of Bacillus cereus, Bacillus thuringiensis and/or Bacillus anthrasis comprising:
- a nutrient base comprising one or more members of the group casein digest, soytone, proteose peptone, meat extract powder, and yeast extract;
- a first chromogenic substrate that changes to a first color responsive to the presence of PC-PLC enzymes comprising 5-bromo-4-chloro-3-indoxyl-choline phosphate;
- a second chromogenic substrate that changes to a second color responsive to the presence of PI-PLC enzymes comprising 5-bromo-4-chloro-3-indoxyl-myo-inositol-1-phosphate;
- the first and second colors blending to a third color that contrasts with the first and second colors, an ingredient that promotes the expression of the PC-PLC and PI-PLC enzymes comprising one or more members of the class bovine serum, silicates, and manganese chloride;
- sodium pyruvate;
- and agar to solidify the mixture.
A plating medium for the presumptive identification of Bacillus cereus, Bacillus thuringiensis and/or Bacillus anthrasis comprising a nutrient base to facilitate growth of Bacillus cereus, Bacillus thuringiensis and/or Bacillus anthrasis, a chromogenic substrate that changes color responsive to the presence of PC-PLC enzymes, and an ingredient that promotes the expression of PC-PLC enzymes.
A plating medium for the presumptive identification of Bacillus cereus, Bacillus thuringiensis and/or Bacillus anthrasis comprising a nutrient base to facilitate growth of Bacillus cereus, Bacillus thuringiensis and Bacillus anthrasis, a chromogenic substrate that changes color responsive to the presence of PI-PLC enzymes, and an ingredient that promotes the expression of PI-PLC enzymes.
Dependencies are inferred from the claim text.