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US 2004/0005650 A1Published application
Caspase 9 activation and uses therefor
AssigneeWyeth
InventorWade A. Edris
Life of this patent
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Abstract
The present invention discloses methods for activating Caspase 9 in such a way that it can be used in assays to discover modulators of Caspase 9.
Claims20
A method for identifying a compound that modulates Caspase 9 activity, compromising:
- a) contacting a sample containing Caspase 9 or a fragment with enzymatic activity thereof with a test compound and a Kosmotropic agent;
- and
- b) detecting the activity of Caspase 9 or the fragment, wherein a change in activity indicates a compound which modulates Caspase 9 activity.
A method for identifying an inhibitor or enhancer of Caspase 9 processing, comprising:
- a) contacting a sample containing proCaspase 9 or a cleavage site containing fragment thereof with protein capable of processing proCaspase 9 and at least one candidate inhibitor or candidate enhancer;
- and
- b) detecting the presence of large and small Caspase 9 subunits, and therefrom determining the level of proCaspase 9 processing, wherein a decrease in processing indicates the presence of a caspase processing inhibitor, and wherein an increase in processing indicates the presence of a caspase processing enhancer.
Dependencies are inferred from the claim text.