Method and composition for detection and treatment of breast cancer
OwnerYan A. Su, Jun Yanginventors
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Abstract
The present invention provides a method for the detection of breast cancer using breast by measuring expression levels of breast cancer specific marker (BCSM) genes, and in particular the level of polynucleotides transcribed from and polypeptides encoded by the BCSM genes. The present invention also provide a method for the treatment and/or prevention of breast cancer by modulating the activity of BCSM genes or the products of BCSM genes.
Claims20
A method for detecting breast cancer in a subject, said method comprising the steps of:
- (a) contacting a biological sample from the subject with an agent that binds to a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38;
- (b) determining a level of binding of said agent to said polypeptide;
- (c) comparing the level of binding of said agent to said polypeptide to a control level of binding;
- and
- (d) producing a diagnosis based on a result from step (c).
A method for detecting breast cancer in a subject, said method comprising the steps of:
- (a) determining a level of a transcribed polynucleotide in a biological sample from said subject, wherein said transcribed polynucleotide comprises a nucleic acid sequence recited in any one of SEQ ID NOS:1-19, or a complement of any of the foregoing nucleic acid sequences;
- (b) comparing the level of said transcribed polynucleotide in said biological sample to a control level of said transcribed polynucleotide;
- and
- (c) producing a diagnosis based on a result from step (b).
A method for detecting breast cancer in a subject, said method comprising the steps of:
- (a) determining an expression pattern of two or more breast cancer-specific markers in a biological sample from said subject;
- (b) comparing the expression pattern of the two or more breast cancer-specific markers in said biological sample to a control expression pattern;
- and
- (c) producing a diagnosis based on a result from step (b),
- wherein said breast cancer-specific marker is a polynucleotide comprising a nucleic acid sequence recited in any one of SEQ ID NOS:1-19 or a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38.
A pharmaceutical composition for preventing or treating breast cancer, comprising pharmaceutically acceptable carrier and an agent capable of modulating an activity of a breast cancer-specific marker or an expression level of a breast cancer-specific gene,
- wherein said breast cancer-specific marker is a polynucleotide comprising a nucleic acid sequence recited in any one of SEQ ID NOS:1-19 or a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38, and
- wherein said breast cancer-specific gene is any one of the genes listed in Tables 4 and 5.
A method of identifying an agent capable of binding to a breast cancer-specific marker, said method comprising:
- contacting a breast cancer-specific marker with a candidate agent;
- and
- determining a binding affinity of said candidate agent to said breast cancer-specific marker,
- wherein said breast cancer-specific marker is a polynucleotide comprising a nucleic acid sequence recited in any one of SEQ ID NOS:1-19 or a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38.
A method of identifying an agent capable of modulating an activity of a breast cancer-specific marker, comprising:
- contacting a breast cancer-specific marker with a candidate agent;
- determining an activity of said breast cancer-specific marker in the presence of said candidate agent;
- determining the activity of said breast cancer-specific marker in the absence of said candidate agent;
- and
- determining whether said candidate agent affects the activity of said breast cancer-specific marker,
- wherein said breast cancer-specific marker is a polynucleotide comprising a nucleic acid sequence recited in any one of SEQ ID NOS:1-19 or a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38.
A biochip comprising any one of:
- (a) a polynucleotide comprising a nucleic acid sequence recited in any one of SEQ ID NOS:1-19;
- (b) a variant of the polynucleotides of (a);
- (c) a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38;
- and
- (d) a variant of the polypeptide of (c),
- wherein the biochip is utilized for diagnosing breast cancer or screening agents that inhibit breast cancer.
A kit for diagnosing breast cancer, said kit comprising a polynucleotide probe or an antibody, wherein said polynucleotides probe specifically binds to a transcribed polynucleotide comprising a nucleic acid sequence recited in any one of SEQ ID NOS:1-19, and wherein said antibody is capable of immunospecific binding to a polypeptide comprising an amino acid sequence recited in any one of SEQ ID NOS:20-38.
Dependencies are inferred from the claim text.