Immunoassay, reagent for immunoassay, and production method of the same
AssigneeCanon Kabushiki Kaisha
InventorsTsutomu Honma, Tetsuya Yano, Tsuyoshi Nomoto, Shinya Kozaki
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Abstract
An assay method for determining presence of a target antibody or antigen in a specimen qualitatively or quantitatively by mixing the specimen with an antigen or antibody immunologically reactive with the target antibody or antigen, and assaying the level of the immunological agglutination reaction, wherein the reactive antigen or antibody is effectively immobilized on the carrier via an amino acid sequence capable of binding to the carrier.
Claims12
An assay method for determining a presence or an amount of a target antibody or antigen in a specimen comprising the steps of:
- preparing a carrier on which an antigen or antibody corresponding to the target antibody or antigen in said specimen respectively is immobilized;
- mixing said carrier with said specimen;
- and
- assaying the level of an immunological agglutination reaction caused by said mixing step,
- wherein said antigen or antibody immobilized on said carrier is bound to said carrier via an amino acid sequence capable of binding to said carrier.
An assay reagent for assaying the presence or amount of a target antibody or antigen in a specimen,
- wherein an antigen or antibody corresponding to said antibody or antigen is immobilized on a carrier, and said antigen or antibody immobilized on said carrier is bound to said carrier via an amino acid sequence capable of binding to said carrier.
A method of producing an assay reagent for assaying the presence or amount of a target antibody or antigen in a specimen,
- which comprises a step of immobilizing an antigen or antibody corresponding to said antibody or antigen on a carrier,
- wherein the antigen or antibody immobilized on said carrier is bound to said carrier via an amino acid sequence capable of binding to said carrier.
A peptide comprising at least one amino acid sequence selected from a group consisting of:
- 13 Lys-Tyr-Asp-Ser-Arg-His-Leu-His-Thr-His-Ser-His, (SEQ ID NO 1) Pro-Asn-Arg-Leu-Gly-Arg-Arg-Pro-Val-Arg-Trp-Glu, (SEQ ID NO 2) Lys-Cys-Cys-Tyr-Tyr-Asp-His-Ser-His-Ala-Leu-Ser, (SEQ ID NO 3) Glu-Tyr-Leu-Ser-Ala-Ile-Val-Ala-Gly-Pro-Trp-Pro, (SEQ ID NO 4) Lys-Leu-Trp-Ile-Leu-Glu-Pro-Thr-Val-Thr-Pro-Thr, (SEQ ID NO 5) Gln-Ser-Asn-Leu-Lys-Val-Ile-Pro-Ser-Trp-Trp-Phe, (SEQ ID NO 6) Trp-Ile-Pro-Pro-Gln-Trp-Ser-Arg-Leu-Ile-Glu-Pro, (SEQ ID NO 7) Asp-His-Pro-Gln-Ala-Lys-Pro-Asn-Trp-Tyr-Gly-Val, (SEQ ID NO 8) Gly-Leu-Pro-Pro-Tyr-Ser-Pro-His-Arg-Leu-Ala-Gln, (SEQ ID NO 9) Lys-Leu-Thr-Thr-Gln-Tyr-Met-Ala-Arg-Ser-Ser-Ser, (SEQ ID NO 10) Lys-Val-Trp-Met-Leu-Pro-Pro-Leu-Pro-Gln-Ala-Thr, (SEQ ID NO 11) Asn-Val-Thr-Ser-Thr-Ala-Phe-Ile-Asp-Thr-Pro-Trp, (SEQ ID NO 12) Arg-Leu-Asn-Leu-Asp-Ile-Ile-Ala-Val-Thr-Ser-Val, (SEQ ID NO 13) Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-Leu-Thr-Val-Val, (SEQ ID NO 14) Thr-Asn-Arg-His-Asn-Pro-His-His-Leu-His-His-Val, (SEQ ID NO 15) Trp-Pro-His-Ala-Trp-Lys-Val-Trp-Trp-Pro-Ala-Ser, (SEQ ID NO 16) Asn-Trp-Trp-Trp-Pro-Pro-Tyr-Ile-Arg-His-Gln-Pro, (SEQ ID NO 17) Trp-His-Trp-Ser-Trp-Thr-Pro-Trp-Pro-Ser-His-His, (SEQ ID NO 18) Trp-Pro-Trp-Ala-Trp-His-Pro-Ser-Arg-Asp-Val-Tyr, (SEQ ID NO 19) Trp-His-Gly-Tyr-Trp-Tyr-Ser-Asn-Leu-Asn-Thr-Thr, (SEQ ID NO 20) Trp-Trp-Thr-Pro-Trp-Met-Ser-His-Ala-Tyr-Pro-Val, (SEQ ID NO 21) Trp-Pro-Asn-Pro-Tyr-Trp-Gly-Trp-Phe-Ala-Ala-Val, (SEQ ID NO 22) Thr-Ser-Trp-His-Thr-Trp-Trp-Trp-Arg-Gln-Pro-Pro, (SEQ ID NO 23) Asn-Ala-Trp-His-Lys-Tyr-Trp-Trp-Pro-Ile-Thr-Lys, (SEQ ID NO 24) His-Pro-Asn-Asn-Asp-Trp-Ser-Lys-Ala-Pro-Gln-Phe, (SEQ ID NO 25) Trp-Trp-Thr-Pro-Gln-Pro-Trp-Trp-Ser-Phe-Pro-Ile, (SEQ ID NO 26) Trp-Pro-His-Thr-Ser-Trp-Trp-Gln-Thr-Pro-Leu-Thr, (SEQ ID NO 27) Trp-His-Val-Asn-Trp-Asp-Pro-Met-Ala-Trp-Tyr-Arg, (SEQ ID NO 28) Ser-Trp-Pro-Trp-Trp-Thr-Ala-Tyr-Arg-Val-His-Ser, (SEQ ID NO 29) Trp-His-Ser-Asn-Trp-Tyr-Gln-Ser-Ile-Pro-Gln-Val, (SEQ ID NO 30) Gly-Tyr-Trp-Pro-Trp-Lys-Phe-Glu-His-Ala-Thr-Val, (SEQ ID NO 31) Ala-Trp-Trp-Pro-Thr-Thr-Phe-Pro-Pro-Tyr-Tyr-Tyr, (SEQ ID NO 32) Asn-Pro-Trp-Trp-Ser-His-Tyr-Tyr-Pro-Arg-Ser-Val, (SEQ ID NO 33) Trp-Pro-His-Asn-Tyr-Pro-Leu-Asn-His-Ser-Asn-Pro, (SEQ ID NO 34) Thr-Trp-Ala-His-Pro-Leu-Glu-Ser-Asp-Tyr-Leu-Arg, (SEQ ID NO 35) His-Thr-Tyr-Tyr-His-Asp-Gly-Trp-Arg-Leu-Ala-Pro, (SEQ ID NO 36) Thr-Phe-Val-Gln-Thr-Pro-Leu-Ser-His-Leu-Ile-Ala, (SEQ ID NO 37) Arg-Val-Pro-Pro-Ser-Lys-Leu-Thr-Arg-Pro-Pro-Phe, (SEQ ID NO 38) His-Ser-Ile-Tyr-Ser-Val-Thr-Pro-Ser-Thr-Ala-Ser, (SEQ ID NO 39) Leu-Asn-Thr-Gln-Asn-His-Ala-Pro-Leu-Pro-Ser-Ile. (SEQ ID NO 40)
Dependencies are inferred from the claim text.