Method for obtaining the binding affinities of a peptide library to a protein

Abstract

The present invention provides a method for measuring the binding of a peptide library to a target protein, both in the presence and absence of a ligand, or other activation modifier. The peptide library is chosen from known binding partners of the target protein, or members of the family to which it belongs. The members of the peptide library include a conserved interaction motif that permits them to bind to the target protein or its family. Individual peptides from the peptide library and the target protein are contacted with one another and a binding affinity measured. The binding affinities across the library are treated as a “fingerprint”. The method is preferably applied to a library of co-regulatory peptides that bind to a nuclear hormone receptor. The present invention further comprises the peptide library, and a composition comprising a member of the peptide library in contact with the target protein.

Dependencies are inferred from the claim text.