Assay
OwnerJonathan Robert Lamb, Gerard Francis Hoyne +2inventors
InventorsJonathan Robert Lamb, Gerard Francis Hoyne, Margaret Jane Dallman, Brian Robert Champion
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Abstract
A method for monitoring the immune system comprising monitoring the Notch signalling pathway.
Claims73
A method for monitoring the immune system comprising a step of detecting modulation of Notch signalling.
A method for monitoring the immune system comprising a step of detecting cleavage of the cleavable intracellular domain of Notch.
A method for monitoring the immune system comprising a step of detecting interaction of Suppresser of Hairless (Su(H)), or homologues thereof, with Notch.
A method for monitoring the immune system comprising a step of detecting interaction of Deltex, or homologues thereof, with Notch.
A method for monitoring the immune system comprising a step of detecting interaction of Deltex, or homologues thereof, with Grb2.
A method for monitoring the immune system comprising a step of detecting modulation of the Ras-JnK signalling pathway.
A method for monitoring the immune system comprising a step of detecting cleavage of the cleavable extracellular domain of Delta.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting modulation of Notch signalling.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting cleavage of the cleavable intracellular domain of Notch.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting interaction of Suppresser of Hairless (Su(H)), or homologues thereof, with Notch.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting interaction of Deltex, or homologues thereof, with Notch.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting interaction of Deltex, or homologues thereof, with Grb2.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting modulation of the Ras-JnK signalling pathway.
A method for detecting or monitoring T-cell activation or inactivation comprising a step of detecting cleavage of the cleavable extracellular domain of Delta.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting modulation of Notch signalling.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting cleavage of the cleavable intracellular domain of Notch.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting interaction of Suppresser of Hairless (Su(H)), or homologues thereof, with Notch.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting interaction of Deltex, or homologues thereof, with Notch.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting interaction of Deltex, or homologues thereof, with Grb2.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting modulation of the Ras-JnK signalling pathway.
A method for detecting or monitoring immunological tolerance or activity comprising a step of detecting cleavage of the cleavable extracellular domain of Delta.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting modulation of Notch signalling.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting cleavage of the cleavable intracellular domain of Notch.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting interaction of Suppresser of Hairless (Su(H)), or homologues thereof, with Notch.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting interaction of Deltex, or homologues thereof, with Notch.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting interaction of Deltex, or homologues thereof, with Grb2.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting modulation of the Ras-JnK signalling pathway.
A method for monitoring the efficacy of immunotherapy comprising a step of detecting cleavage of the cleavable extracellular domain of Delta.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising a step of detecting modulation of Notch signalling.
A method for detecting or monitoring Notch signalling comprising a step of detecting modulation of Notch signalling.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising a step of detecting cleavage of the cleavable intracellular domain of Notch.
A method for detecting or monitoring Notch signalling comprising a step of detecting cleavage of the cleavable intracellular domain of Notch.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising a step of detecting interaction of Suppresser of Hairless (Su(H)), or homologues thereof, with Notch.
A method for detecting or monitoring Notch signalling comprising a step of detecting interaction of Suppresser of Hairless (Su(H)), or homologues thereof, with Notch.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising a step of detecting interaction of Deltex, or homologues thereof, with Notch.
A method for detecting or monitoring Notch signalling comprising a step of detecting interaction of Deltex, or homologues thereof, with Notch.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising a step of detecting interaction of Deltex, or homologues thereof, with Grb2.
A method for detecting or monitoring Notch signalling comprising a step of detecting interaction of Deltex, or homologues thereof, with Grb2.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising a step of detecting modulation of the Ras-JnK signalling pathway.
A method for detecting or monitoring Notch signalling comprising a step of detecting modulation of the Ras-JnK signalling pathway.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising the steps of:
- a. providing a T-cell in the presence of an antigen;
- b. detecting modulation of Notch signalling within the T-cell;
- and
- c. determining the reactivity of the T-cell to said antigen.
A method according to any preceding claim further comprising a step of comparing detectable Notch signalling with a reference signal.
A method according to any preceding claim wherein modulation of Notch signalling is detected using a nucleic acid assay.
A method according to any preceding claim comprising a step of detecting modulation of the expression of a target gene of Notch signalling.
A method for detecting or monitoring the reactivity of a T-cell to an antigen comprising the steps of:
- a. providing a ligand capable of binding to Notch such as to modulate Notch signalling;
- b. providing a T-cell having a Notch receptor or a functional variant thereof capable of transducing Notch signalling;
- c. allowing the ligand to bind to Notch in the presence of an antigen;
- d. detecting modulation of Notch signalling;
- and
- e. determining the reactivity of the T-cell to said antigen using a method according to any preceding claim.
A method according to any preceding claim for detecting increased reactivity of a T-cell to an antigen.
A method according to any preceding claim wherein the antigen is a self antigen.
A kit for detecting or monitoring modulation of Notch signalling comprising a plurality of sequences or binding agents specific to nucleic acid or protein targets of Notch signalling.
A nucleic acid or protein array consisting essentially of a plurality of sequences or binding agents specific to nucleic acid or protein targets of Notch signalling.
The use of a detectable signal of Notch signalling for detecting or monitoring the reactivity of a T-cell to an antigen.
The use of a detectable signal of Notch signalling for monitoring the immune system.
The use of a detectable signal of Notch signalling for detecting or monitoring T-cell activation or inactivation.
The use of a detectable signal of Notch signalling for detecting or monitoring immunological tolerance or activation.
Dependencies are inferred from the claim text.