Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
AssigneePioneer Hi-Bred International, Inc.
InventorsChristopher L. Baszczynski, Benjamin A. Bowen, David J. Peterson, Laura Tagliani
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Abstract
Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
Claims22
A method for targeting the insertion of a polynucleotide of interest to a specific chromosomal site in a genome of a plant cell, said method comprising:
- a) introducing into the plant cell a transfer cassette, said transfer cassette comprising said polynucleotide of interest flanked by a first and a second recombination site, wherein said first and said second recombination sites are non-identical;
- and, wherein said genome of said plant cell comprises a target site comprising the first and the second non-identical recombination sites of said transfer cassette;
- and,
- b) providing a recombinase that recognizes and implements recombination at the first and the second recombination sites.
Dependencies are inferred from the claim text.