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US 2004/0003433 A1Published application
Cell cycle nucleic acids, polypeptides and uses thereof
AssigneesPioneer Hi-Bred International, Inc., Arizona Board of Regents on Behalf of the University of Arizona
InventorsWilliam J. Gordon-Kamm, Keith S. Lowe, Brian A. Larkins, Brian R. Dilkes, Yuejin Sun
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Abstract
The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression, cell number and/or composition of plants.
Claims78
An isolated nucleic acid comprising:
- a) a polynucleotide that encodes a polypeptide of SEQ ID NO:
- 2, 4, or 6;
- b) a polynucleotide amplified from a Zea mays nucleic acid library using the primers from Seq ID NO:
- 1, 3 or 5;
- c) a polynucleotide amplified from a Zea mays nucleic acid library using a 5′ primer comprising ATG GGN AAR TAY ATG (CGN or AGR) AAR and a 3′ primer comprising Seq ID NO:
- 1, 3 or 5;
- d) a polynucleotide amplified from a Zea mays nucleic acid library using a 5′ primer comprising ATG GGN AAR TAY ATG (CGN or AGR) MR and a 3′ primer comprising the CDK binding region of Seq ID NO:
- 1, 3 or 5;
- e) a polynucleotide comprising at least 20 contiguous bases of SEQ ID NO:
- 1, 3 or 5;
- f) a polynucleotide comprising at least 20 contiguous bases of SEQ ID NO:
- 1, 3 or 5 and ATG GGN MR TAY ATG (CGN or AGR) MR;
- g) a polynucleotide comprising at least 20 contiguous bases of the 3′ coding region of SEQ ID NO:
- 1, 3 or 5;
- h) a polynucleotide comprising at least 20 contiguous bases of the 3′ coding region of SEQ ID NO:
- 1, 3 or 5 and at least some of the CDK binding region;
- i) a polynucleotide encoding a maize CKI protein;
- j) a polynucleotide having at least 82% sequence identity to SEQ ID NOS:
- 1, 3, or 5 wherein the % sequence identity is based on the entire coding sequence of SEQ ID NOS:
- 1, 3, or 5 and is determined by BLAST 2.0, using default parameters;
- k) a polynucleotide comprising at least 25 nucleotides in length which hybridizes under low stringency conditions to a polynucleotide having the sequence set forth in SEQ ID NO:
- 1, 3 or 5, wherein the conditions include hybridization with a buffer solution of 30% formamide, 1 M NaCl, 1% SDS at 37° C. and a wash in 2×SSC at 50° C.;
- l) a polynucleotide comprising at least 25 nucleotides in length and is coding sequence which hybridizes under low stringency conditions to a polynucleotide having the sequence set forth in SEQ ID NO:
- 1, 3 or 5, wherein the conditions include hybridization with a buffer solution of 30% formamide, 1 M NaCl, 1% SDS at 37° C. and a wash in 2×SSC at 50° C.;
- m) a polynucleotide encoding a polypeptide having CKI activity comprising a polynucleotide having at least 68% sequence identity to SEQ ID NO.:
- 3, wherein the wherein the polynucleotide is other than from AC069145, and wherein the percent sequence identity is based on the sequence comprising the amino acid coding sequence and is determined by BLAST 2.0 using default parameters;
- n) a maize polynucleotide encoding a polypeptide having CKI activity comprising a polynucleotide having at least 68% sequence identity to SEQ ID NO.:
- 3 and wherein the percent sequence identity is based on the sequence comprising the amino acid coding sequence and is determined by BLAST 2.0 using default parameters;
- o) a polynucleotide encoding a polypeptide having CKI activity and at least 73% sequence identity to SEQ ID NO.:
- 4, wherein the wherein the polynucleotide is other than from AC069145, and wherein the percent sequence identity is based on the amino acid coding sequence and is determined by BLAST 2.0 using default parameters;
- p) a polynucleotide complementary to a polynucleotide of (a) through (O).
28 more elements
A method of modulating the activity of CDK in a plant comprising;
- a) stably transforming a plant cell with a maize CKI polynucleotide operably linked to a promoter, wherein the polynucleotide is in sense or antisense orientation;
- b) growing the plant cell under plant growing conditions to produce a regenerated plant capable of expressing the CKI expression product for a time sufficient to modulate CDK activity in the plant.
A method for modulating the expression of CKI in plant cells comprising:
- a) introducing into a plant cell one or more interactors;
- and
- b) expressing the interactor for a time sufficient to modulate CKI expression in the plant cell.
A method for modulating the activity of CKI in plant cells comprising introducing into the plant cells one or more interactors capable of expressing the interactor for a time sufficient to modulate CKI activity in the plant cell.
A method for ectopic expression to modulate cell proliferation comprising:
- a) stably transforming a plant cell with an maize CKI polynucleotide operably linked to a promoter, wherein the polynucleotide is in sense or antisense orientation;
- b) growing the plant cell under plant growing conditions to produce a regenerated plant capable of ectopic expression of the maize CKI polynucleotide for a time sufficient to modulate cell proliferation in the plant.
A method for improving transformation comprising:
- a) stably transforming a plant cell with a maize CKI polynucleotide operably linked to a promoter, wherein the polynucleotide is in sense or antisense orientation;
- b) growing the transformed plant cell under plant growing conditions to produce a regenerated plant cell capable of expressing the CKI expression product for a time sufficient to modulate CDK activity in the transformed plant cell;
- and
- c) transforming the transformed plant cell with a second polynucleotide operably linked to a promoter, wherein the polynucleotide is in sense or antisense orientation to produce a second transformed plant cell.
1 more element
A method for improving transformation comprising:
- a) introducing into a plant cell one or more interactors;
- b) expressing one or more interactors for a time sufficient to modulate CKI expression in the plant cell;
- c) transforming the transformed plant cell with a polynucleotide operably linked to a promoter, wherein the polynucleotide is in sense or antisense orientation to produce a transformed plant cell;
- and
- d) growing the transformed plant cell under plant growing conditions to produce a regenerated plant.
2 more elements
Dependencies are inferred from the claim text.