Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose
AssigneeWeyerhaeuser NR Company
InventorsPramod K. Gupta, Diane Holmstrom, Bonnie Larson
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Abstract
The present invention provides methods for producing cotyledonary pine embryos. The methods of the invention each include the step of culturing embryogenic pine tissue in, or on, a medium comprising a disaccharide and glucose to yield cotyledonary pine embryos, wherein the disaccharide and glucose are each present in the medium at a concentration of less than 3 percent. The present invention also provides cotyledonary pine embryos prepared by a method of the invention.
Claims33
A method for producing cotyledonary pine embryos, said method comprising the step of culturing embryogenic pine tissue in, or on, a medium comprising a disaccharide and glucose to yield cotyledonary pine embryos, wherein said disaccharide and said glucose are each present in the medium at a concentration of less than 3 percent.
A method for producing cotyledonary pine embryos, said method comprising the steps of:
- (a) culturing embryogenic pine tissue in, or on, a maintenance medium;
- and
- (b) culturing the embryogenic pine tissue treated in accordance with step (a) in, or on, a development medium comprising glucose and a disaccharide, to form cotyledonary pine embryos, wherein the disaccharide and glucose are each present in the development medium at a concentration of less than 3 percent.
Dependencies are inferred from the claim text.